A systematic review of methods used to sample and analyse periradicular tissue fluid during root canal treatment

S S Virdee; K Butt; M Grant; J Camilleri; P R Cooper; P L Tomson

doi:10.1111/iej.13104

A systematic review of methods used to sample and analyse periradicular tissue fluid during root canal treatment

S S Virdee, K Butt, M Grant, J Camilleri, P R Cooper, P L Tomson

Research output: Contribution to journal › Review article › peer-review

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Abstract

AIM: The primary aim was to identify techniques used to sample and analyse Periradicular Tissue Fluid (PTF) in permanent teeth diagnosed with apical disease during root canal treatment. Secondly, to identify the types of inflammatory mediators studied using this approach.

METHODOLOGY:

DATA SOURCES: PubMed, EMBASE, Cochrane Library, Science Direct, Web of Science and OpenGrey.

ELIGIBILITY CRITERIA: Clinical studies published until 1st June 2018 which utilised orthograde techniques to sample and analyse PTF were included. Cell culture, laboratory or animal studies and those concerned with investigating inflammatory mediator activity from within healthy or diseased pulp tissue, and not periradicular tissues, were excluded.

STUDY APPRASIAL & METHODS: In accordance with PRISMA guidelines, data was extracted on study characteristics, target mediators, sampling and assay techniques and the parameters associated with the PTF sampling and eluting protocol. A qualitative synthesis was conducted and studies were critically appraised using a modified version of the Cochrane risk of bias tool.

RESULTS:

STUDY CHARACTERSITCS: From 251 studies, 33 were eligible for inclusion. Sampling techniques included the use of paper points (n=27), fine needle aspiration (n=4) and filter strips (n=2). Assay techniques included Enzyme-linked-immunosorbant-assay (n=18), quantitative polymerase chain reaction (n=9), radioimmunoassay (n=4), colorimetric-assay (n=2), immunofluorometric-assay (n=1) and cytometric-bead-array (n=1). Forty-five different inflammatory mediators were targeted at the proteomic/metabolomic (n=25) or transcriptomic level (n=9).

LIMITATIONS: Significant heterogeneity exists within the methodology and only 5 studies disclosed unambiguous information about their PTF sampling and eluting protocols.

CONCLUSIONS: Paper points and proteomic/metabolomic analysis are currently the preferred methods for studying and analysing PTF during NSRCT. The most studied analytes were IL-1β and TNF-α.

IMPLICATIONS: Further research is required to develop an optimised PTF sampling and eluting protocol to overcome methodological heterogeneity and future studies are advised to follow a standardised approach to reporting data. This article is protected by copyright. All rights reserved.

Original language	English
Pages (from-to)	1108-1127
Number of pages	20
Journal	International Endodontic Journal
Volume	52
Issue number	8
Early online date	25 Feb 2019
DOIs	https://doi.org/10.1111/iej.13104
Publication status	Published - Aug 2019

Bibliographical note

Keywords

Endodontics
periradicular tissue fluid
cytokine
inflammation
sampling
systematic review

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10.1111/iej.13104Licence: None: All rights reserved

S_S_Virdee_et_al_A_systematic_review_of_methods_International_Endodontic_Journal_2019
Checked for eligibility: 04/03/2019 This is the peer reviewed version of the following article: Virdee, S. S., Butt, K. , Grant, M. , Camilleri, J. , Cooper, P. R. and Tomson, P. L. (2019), A systematic review of methods used to sample and analyse periradicular tissue fluid during root canal treatment. Int Endod J., which has been published in final form at: https://doi.org/10.1111/iej.13104. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Accepted author manuscript, 751 KBLicence: Other (please specify with Rights Statement)

Cite this

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title = "A systematic review of methods used to sample and analyse periradicular tissue fluid during root canal treatment",

abstract = "AIM: The primary aim was to identify techniques used to sample and analyse Periradicular Tissue Fluid (PTF) in permanent teeth diagnosed with apical disease during root canal treatment. Secondly, to identify the types of inflammatory mediators studied using this approach.METHODOLOGY: DATA SOURCES: PubMed, EMBASE, Cochrane Library, Science Direct, Web of Science and OpenGrey.ELIGIBILITY CRITERIA: Clinical studies published until 1st June 2018 which utilised orthograde techniques to sample and analyse PTF were included. Cell culture, laboratory or animal studies and those concerned with investigating inflammatory mediator activity from within healthy or diseased pulp tissue, and not periradicular tissues, were excluded.STUDY APPRASIAL & METHODS: In accordance with PRISMA guidelines, data was extracted on study characteristics, target mediators, sampling and assay techniques and the parameters associated with the PTF sampling and eluting protocol. A qualitative synthesis was conducted and studies were critically appraised using a modified version of the Cochrane risk of bias tool.RESULTS: STUDY CHARACTERSITCS: From 251 studies, 33 were eligible for inclusion. Sampling techniques included the use of paper points (n=27), fine needle aspiration (n=4) and filter strips (n=2). Assay techniques included Enzyme-linked-immunosorbant-assay (n=18), quantitative polymerase chain reaction (n=9), radioimmunoassay (n=4), colorimetric-assay (n=2), immunofluorometric-assay (n=1) and cytometric-bead-array (n=1). Forty-five different inflammatory mediators were targeted at the proteomic/metabolomic (n=25) or transcriptomic level (n=9).LIMITATIONS: Significant heterogeneity exists within the methodology and only 5 studies disclosed unambiguous information about their PTF sampling and eluting protocols.CONCLUSIONS: Paper points and proteomic/metabolomic analysis are currently the preferred methods for studying and analysing PTF during NSRCT. The most studied analytes were IL-1β and TNF-α.IMPLICATIONS: Further research is required to develop an optimised PTF sampling and eluting protocol to overcome methodological heterogeneity and future studies are advised to follow a standardised approach to reporting data. This article is protected by copyright. All rights reserved.",

keywords = "Endodontics, periradicular tissue fluid, cytokine, inflammation, sampling, systematic review",

author = "Virdee, {S S} and K Butt and M Grant and J Camilleri and Cooper, {P R} and Tomson, {P L}",

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AU - Grant, M

AU - Camilleri, J

AU - Cooper, P R

AU - Tomson, P L

PY - 2019/8

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N2 - AIM: The primary aim was to identify techniques used to sample and analyse Periradicular Tissue Fluid (PTF) in permanent teeth diagnosed with apical disease during root canal treatment. Secondly, to identify the types of inflammatory mediators studied using this approach.METHODOLOGY: DATA SOURCES: PubMed, EMBASE, Cochrane Library, Science Direct, Web of Science and OpenGrey.ELIGIBILITY CRITERIA: Clinical studies published until 1st June 2018 which utilised orthograde techniques to sample and analyse PTF were included. Cell culture, laboratory or animal studies and those concerned with investigating inflammatory mediator activity from within healthy or diseased pulp tissue, and not periradicular tissues, were excluded.STUDY APPRASIAL & METHODS: In accordance with PRISMA guidelines, data was extracted on study characteristics, target mediators, sampling and assay techniques and the parameters associated with the PTF sampling and eluting protocol. A qualitative synthesis was conducted and studies were critically appraised using a modified version of the Cochrane risk of bias tool.RESULTS: STUDY CHARACTERSITCS: From 251 studies, 33 were eligible for inclusion. Sampling techniques included the use of paper points (n=27), fine needle aspiration (n=4) and filter strips (n=2). Assay techniques included Enzyme-linked-immunosorbant-assay (n=18), quantitative polymerase chain reaction (n=9), radioimmunoassay (n=4), colorimetric-assay (n=2), immunofluorometric-assay (n=1) and cytometric-bead-array (n=1). Forty-five different inflammatory mediators were targeted at the proteomic/metabolomic (n=25) or transcriptomic level (n=9).LIMITATIONS: Significant heterogeneity exists within the methodology and only 5 studies disclosed unambiguous information about their PTF sampling and eluting protocols.CONCLUSIONS: Paper points and proteomic/metabolomic analysis are currently the preferred methods for studying and analysing PTF during NSRCT. The most studied analytes were IL-1β and TNF-α.IMPLICATIONS: Further research is required to develop an optimised PTF sampling and eluting protocol to overcome methodological heterogeneity and future studies are advised to follow a standardised approach to reporting data. This article is protected by copyright. All rights reserved.

AB - AIM: The primary aim was to identify techniques used to sample and analyse Periradicular Tissue Fluid (PTF) in permanent teeth diagnosed with apical disease during root canal treatment. Secondly, to identify the types of inflammatory mediators studied using this approach.METHODOLOGY: DATA SOURCES: PubMed, EMBASE, Cochrane Library, Science Direct, Web of Science and OpenGrey.ELIGIBILITY CRITERIA: Clinical studies published until 1st June 2018 which utilised orthograde techniques to sample and analyse PTF were included. Cell culture, laboratory or animal studies and those concerned with investigating inflammatory mediator activity from within healthy or diseased pulp tissue, and not periradicular tissues, were excluded.STUDY APPRASIAL & METHODS: In accordance with PRISMA guidelines, data was extracted on study characteristics, target mediators, sampling and assay techniques and the parameters associated with the PTF sampling and eluting protocol. A qualitative synthesis was conducted and studies were critically appraised using a modified version of the Cochrane risk of bias tool.RESULTS: STUDY CHARACTERSITCS: From 251 studies, 33 were eligible for inclusion. Sampling techniques included the use of paper points (n=27), fine needle aspiration (n=4) and filter strips (n=2). Assay techniques included Enzyme-linked-immunosorbant-assay (n=18), quantitative polymerase chain reaction (n=9), radioimmunoassay (n=4), colorimetric-assay (n=2), immunofluorometric-assay (n=1) and cytometric-bead-array (n=1). Forty-five different inflammatory mediators were targeted at the proteomic/metabolomic (n=25) or transcriptomic level (n=9).LIMITATIONS: Significant heterogeneity exists within the methodology and only 5 studies disclosed unambiguous information about their PTF sampling and eluting protocols.CONCLUSIONS: Paper points and proteomic/metabolomic analysis are currently the preferred methods for studying and analysing PTF during NSRCT. The most studied analytes were IL-1β and TNF-α.IMPLICATIONS: Further research is required to develop an optimised PTF sampling and eluting protocol to overcome methodological heterogeneity and future studies are advised to follow a standardised approach to reporting data. This article is protected by copyright. All rights reserved.

KW - Endodontics

KW - periradicular tissue fluid

KW - cytokine

KW - inflammation

KW - sampling

KW - systematic review

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DO - 10.1111/iej.13104

M3 - Review article

C2 - 30802974

SN - 0143-2885

VL - 52

SP - 1108

EP - 1127

JO - International Endodontic Journal

JF - International Endodontic Journal

IS - 8

ER -

A systematic review of methods used to sample and analyse periradicular tissue fluid during root canal treatment

Abstract

Bibliographical note

Keywords

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