Projects per year
Abstract
Context: Non alcoholic fatty liver disease (NAFLD) is the hepatic manifestation of the metabolic syndrome. NAFLD represents a spectrum of liver disease ranging from reversible hepatic steatosis, to non alcoholic steato-hepatitis (NASH) and cirrhosis. The potential role of glucocorticoids (GC) in the pathogenesis of NAFLD is highlighted in patients with GC excess, Cushing's syndrome, who develop central adiposity, insulin resistance and in 20% of cases, NAFLD. Although in most cases of NAFLD, circulating cortisol levels are normal, hepatic cortisol availability is controlled by enzymes that regenerate cortisol (F) from inactive cortisone (E) (11 beta-hydroxysteroid dehydrogenase type 1, 11 beta-HSD1), or inactivate cortisol through A-ring metabolism (5 alpha- and 5 beta-reductase, 5 alpha R and 5 beta R).
Objective and Methods: In vitro studies defined 11 beta-HSD1 expression in normal and NASH liver samples. We then characterised hepatic cortisol metabolism in 16 patients with histologically proven NAFLD compared to 32 obese controls using gas chromatographic analysis of 24 hour urine collection and plasma cortisol generation profile following oral cortisone.
Results: In patients with steatosis 5 alpha R activity was increased, with a decrease in hepatic 11 beta-HSD1 activity. Total cortisol metabolites were increased in this group consistent with increased GC production rate. In contrast, in patients with NASH, 11 beta-HSD1 activity was increased both in comparison to patients with steatosis, and controls. Endorsing these findings, 11 beta-HSD1 mRNA and immunostaining was markedly increased in NASH patients in peri septal hepatocytes and within CD68 positive macrophages within inflamed cirrhotic septa.
Conclusion: Patients with hepatic steatosis have increased clearance and decreased hepatic regeneration of cortisol and we propose that this may represent a protective mechanism to decrease local GC availability to preserve hepatic metabolic phenotype. With progression to NASH, increased 11 beta-HSD1 activity and consequent cortisol regeneration may serve to limit hepatic inflammation.
Original language | English |
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Article number | e29531 |
Pages (from-to) | e29531 |
Journal | PLoS ONE |
Volume | 7 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1 Feb 2012 |
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Dive into the research topics of 'A Switch in Hepatic Cortisol Metabolism across the Spectrum of Non Alcoholic Fatty Liver Disease'. Together they form a unique fingerprint.Projects
- 2 Finished
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Glucocorticoid Metabolism and the Control of Metabolic Phenotype
Tomlinson, J.
1/10/09 → 30/09/14
Project: Research Councils
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The Role of 11 Beta Hydroxysteroid Dehydrogenase Type 1 and Hexose-6-Phosphate Dehydrogenase in the Metabolic Syndrome
Stewart, P.
1/04/05 → 30/09/09
Project: Research Councils