A study of biofilm and non-line-of-sight bio-hydroxyapatite coatings using a Serratia sp.
Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
- University of Cambridge
- Department of Chemical Engineering
This study describes biofilm formation as a non line-of-sight coating method on support materials such as polyurethane foam, porous glass, polypropylene (PP) and titanium alloy, using a Serratia sp., which can manufacture extracellular nanoscale scaffolded hydroxyapatite (HA) crystals via enzymatic cleavage of glycerol 2-phosphate (G2P) in the presence of CaCl 2. Various microscopies and non-invasive magnetic resonance imaging were used to visualize the biofilm coating on the support surface. A novel micromanipulation technique was used to estimate the adhesive strength of native and HA-mineralized biofilms. The biofilm with HA was up to forty times stronger than that without HA. A coating of nano-HA (> 80 μm) onto a biofilm-Ti disc was achieved.
|Title of host publication||Advanced Materials Research|
|Publication status||Published - 2009|
|Event||18th International Biohydrometallurgy Symposium, IBS 2009 - Bariloche, Argentina|
Duration: 13 Sep 2009 → 17 Sep 2009
|Name||Advanced Materials Research|
|Conference||18th International Biohydrometallurgy Symposium, IBS 2009|
|Period||13/09/09 → 17/09/09|
- Biofilm, Biomineralization, Hydroxyapatite, Serratia