A study of biofilm and non-line-of-sight bio-hydroxyapatite coatings using a Serratia sp.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Colleges, School and Institutes

External organisations

  • University of Cambridge
  • Department of Chemical Engineering


This study describes biofilm formation as a non line-of-sight coating method on support materials such as polyurethane foam, porous glass, polypropylene (PP) and titanium alloy, using a Serratia sp., which can manufacture extracellular nanoscale scaffolded hydroxyapatite (HA) crystals via enzymatic cleavage of glycerol 2-phosphate (G2P) in the presence of CaCl 2. Various microscopies and non-invasive magnetic resonance imaging were used to visualize the biofilm coating on the support surface. A novel micromanipulation technique was used to estimate the adhesive strength of native and HA-mineralized biofilms. The biofilm with HA was up to forty times stronger than that without HA. A coating of nano-HA (> 80 μm) onto a biofilm-Ti disc was achieved.


Original languageEnglish
Title of host publicationAdvanced Materials Research
Publication statusPublished - 2009
Event18th International Biohydrometallurgy Symposium, IBS 2009 - Bariloche, Argentina
Duration: 13 Sep 200917 Sep 2009

Publication series

NameAdvanced Materials Research
ISSN (Print)10226680


Conference18th International Biohydrometallurgy Symposium, IBS 2009


  • Biofilm, Biomineralization, Hydroxyapatite, Serratia

ASJC Scopus subject areas