A stroma-derived defect in nfkb2-/- mice causes impaired lymph node development and lymphocyte recruitment

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A stroma-derived defect in nfkb2-/- mice causes impaired lymph node development and lymphocyte recruitment. / Carragher, Damian; Johal, Ramneek; Button, Adele; Eliopoulos, Aristides; Jenkinson, Eric; Anderson, Graham; Caamano, Jorge.

In: Journal of Immunology, Vol. 173, 01.01.2004, p. 2271-79.

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@article{6d6076688cd34c008772640f862066fa,
title = "A stroma-derived defect in nfkb2-/- mice causes impaired lymph node development and lymphocyte recruitment",
abstract = "The NF-kappaB family of transcription factors is vital to all aspects of immune function and regulation in both the hemopoietic and stromal compartments of immune environments. Recent studies of mouse models deficient for specific members of the NF-kappaB family have revealed critical roles for these proteins in the process of secondary lymphoid tissue organogenesis. In this study, we investigate the role of NF-kappaB family member NF-kappaB2 in lymph node development and lymphocyte recruitment. Inguinal lymph nodes in nfkappab2(-/-) mice are reduced in size and cellularity, most notably in the B cell compartment. Using in vitro and in vivo lymph node grafting assays, we show that the defect resides in the stromal compartment. Further examination of the nfkappab2(-/-) inguinal lymph nodes revealed that expression of peripheral node addressin components CD34 and glycosylation-dependent cell adhesion molecule-1 along with the high endothelial venule-restricted sulfoltransferase HEC-GlcNAc6ST was markedly reduced. Furthermore, expression of the lymphocyte homing chemokines CCL19, CCL21, and CXCL13 was down-regulated. These data highlight the role of NF-kappaB2 in inguinal lymph node organogenesis and recruitment of lymphocytes to these organs due to its role in up-regulation of essential cell adhesion molecules and chemokines, while suggesting a potential role for NF-kappaB2 in organization of lymph node endothelium.",
author = "Damian Carragher and Ramneek Johal and Adele Button and Aristides Eliopoulos and Eric Jenkinson and Graham Anderson and Jorge Caamano",
year = "2004",
month = jan,
day = "1",
language = "English",
volume = "173",
pages = "2271--79",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",

}

RIS

TY - JOUR

T1 - A stroma-derived defect in nfkb2-/- mice causes impaired lymph node development and lymphocyte recruitment

AU - Carragher, Damian

AU - Johal, Ramneek

AU - Button, Adele

AU - Eliopoulos, Aristides

AU - Jenkinson, Eric

AU - Anderson, Graham

AU - Caamano, Jorge

PY - 2004/1/1

Y1 - 2004/1/1

N2 - The NF-kappaB family of transcription factors is vital to all aspects of immune function and regulation in both the hemopoietic and stromal compartments of immune environments. Recent studies of mouse models deficient for specific members of the NF-kappaB family have revealed critical roles for these proteins in the process of secondary lymphoid tissue organogenesis. In this study, we investigate the role of NF-kappaB family member NF-kappaB2 in lymph node development and lymphocyte recruitment. Inguinal lymph nodes in nfkappab2(-/-) mice are reduced in size and cellularity, most notably in the B cell compartment. Using in vitro and in vivo lymph node grafting assays, we show that the defect resides in the stromal compartment. Further examination of the nfkappab2(-/-) inguinal lymph nodes revealed that expression of peripheral node addressin components CD34 and glycosylation-dependent cell adhesion molecule-1 along with the high endothelial venule-restricted sulfoltransferase HEC-GlcNAc6ST was markedly reduced. Furthermore, expression of the lymphocyte homing chemokines CCL19, CCL21, and CXCL13 was down-regulated. These data highlight the role of NF-kappaB2 in inguinal lymph node organogenesis and recruitment of lymphocytes to these organs due to its role in up-regulation of essential cell adhesion molecules and chemokines, while suggesting a potential role for NF-kappaB2 in organization of lymph node endothelium.

AB - The NF-kappaB family of transcription factors is vital to all aspects of immune function and regulation in both the hemopoietic and stromal compartments of immune environments. Recent studies of mouse models deficient for specific members of the NF-kappaB family have revealed critical roles for these proteins in the process of secondary lymphoid tissue organogenesis. In this study, we investigate the role of NF-kappaB family member NF-kappaB2 in lymph node development and lymphocyte recruitment. Inguinal lymph nodes in nfkappab2(-/-) mice are reduced in size and cellularity, most notably in the B cell compartment. Using in vitro and in vivo lymph node grafting assays, we show that the defect resides in the stromal compartment. Further examination of the nfkappab2(-/-) inguinal lymph nodes revealed that expression of peripheral node addressin components CD34 and glycosylation-dependent cell adhesion molecule-1 along with the high endothelial venule-restricted sulfoltransferase HEC-GlcNAc6ST was markedly reduced. Furthermore, expression of the lymphocyte homing chemokines CCL19, CCL21, and CXCL13 was down-regulated. These data highlight the role of NF-kappaB2 in inguinal lymph node organogenesis and recruitment of lymphocytes to these organs due to its role in up-regulation of essential cell adhesion molecules and chemokines, while suggesting a potential role for NF-kappaB2 in organization of lymph node endothelium.

M3 - Article

VL - 173

SP - 2271

EP - 2279

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

ER -