A role in cell-binding for the C-terminus of pneumolysin, the thiol-activated toxin of Streptococcus pneumoniae

R H Owen; G J Boulnois; P W Andrew; T J Mitchell

A role in cell-binding for the C-terminus of pneumolysin, the thiol-activated toxin of Streptococcus pneumoniae

R H Owen, G J Boulnois, P W Andrew, T J Mitchell

Microbiology and Infection

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

Cell binding of pneumolysin to target cells is an important step in the lysis of cells by this toxin. We sought to locate the cell-binding region of pneumolysin. Deletion of the six C-terminal amino acids decreased cell-binding activity by 98%. Furthermore, mutagenesis of an amino acid near the C-terminus decreased the cell-binding activity of full-length pneumolysin by 90%. The C-terminus of pneumolysin has an important role in cell-binding activity.

Original language	English
Pages (from-to)	217-21
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	121
Issue number	2
Publication status	Published - 15 Aug 1994

Keywords

Bacterial Proteins
Binding Sites
Cytotoxins
Streptococcus pneumoniae
Streptolysins

Cite this

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title = "A role in cell-binding for the C-terminus of pneumolysin, the thiol-activated toxin of Streptococcus pneumoniae",

abstract = "Cell binding of pneumolysin to target cells is an important step in the lysis of cells by this toxin. We sought to locate the cell-binding region of pneumolysin. Deletion of the six C-terminal amino acids decreased cell-binding activity by 98%. Furthermore, mutagenesis of an amino acid near the C-terminus decreased the cell-binding activity of full-length pneumolysin by 90%. The C-terminus of pneumolysin has an important role in cell-binding activity.",

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author = "Owen, {R H} and Boulnois, {G J} and Andrew, {P W} and Mitchell, {T J}",

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T1 - A role in cell-binding for the C-terminus of pneumolysin, the thiol-activated toxin of Streptococcus pneumoniae

AU - Owen, R H

AU - Boulnois, G J

AU - Andrew, P W

AU - Mitchell, T J

PY - 1994/8/15

Y1 - 1994/8/15

N2 - Cell binding of pneumolysin to target cells is an important step in the lysis of cells by this toxin. We sought to locate the cell-binding region of pneumolysin. Deletion of the six C-terminal amino acids decreased cell-binding activity by 98%. Furthermore, mutagenesis of an amino acid near the C-terminus decreased the cell-binding activity of full-length pneumolysin by 90%. The C-terminus of pneumolysin has an important role in cell-binding activity.

AB - Cell binding of pneumolysin to target cells is an important step in the lysis of cells by this toxin. We sought to locate the cell-binding region of pneumolysin. Deletion of the six C-terminal amino acids decreased cell-binding activity by 98%. Furthermore, mutagenesis of an amino acid near the C-terminus decreased the cell-binding activity of full-length pneumolysin by 90%. The C-terminus of pneumolysin has an important role in cell-binding activity.

KW - Bacterial Proteins

KW - Binding Sites

KW - Cytotoxins

KW - Streptococcus pneumoniae

KW - Streptolysins

M3 - Article

C2 - 7926673

SN - 0378-1097

VL - 121

SP - 217

EP - 221

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2

ER -

A role in cell-binding for the C-terminus of pneumolysin, the thiol-activated toxin of Streptococcus pneumoniae

Abstract

Keywords

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