A quantitative determination of lipid bilayer deposition efficiency using AFM

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A quantitative determination of lipid bilayer deposition efficiency using AFM. / Wood, Mary H.; Milan, David C.; Nichols, Richard J.; Casford, Michael T.L.; Horswell, Sarah L.

In: RSC Advances, Vol. 11, No. 32, 02.06.2021, p. 19768-19778.

Research output: Contribution to journalArticlepeer-review

Harvard

Wood, MH, Milan, DC, Nichols, RJ, Casford, MTL & Horswell, SL 2021, 'A quantitative determination of lipid bilayer deposition efficiency using AFM', RSC Advances, vol. 11, no. 32, pp. 19768-19778. https://doi.org/10.1039/d1ra01920a

APA

Wood, M. H., Milan, D. C., Nichols, R. J., Casford, M. T. L., & Horswell, S. L. (2021). A quantitative determination of lipid bilayer deposition efficiency using AFM. RSC Advances, 11(32), 19768-19778. https://doi.org/10.1039/d1ra01920a

Vancouver

Author

Wood, Mary H. ; Milan, David C. ; Nichols, Richard J. ; Casford, Michael T.L. ; Horswell, Sarah L. / A quantitative determination of lipid bilayer deposition efficiency using AFM. In: RSC Advances. 2021 ; Vol. 11, No. 32. pp. 19768-19778.

Bibtex

@article{93be3890926a4a44903dfb98bb06c860,
title = "A quantitative determination of lipid bilayer deposition efficiency using AFM",
abstract = "The efficacy of a number of different methods for depositing a dimyristoylphosphatidylcholine (DMPC) lipid bilayer or DMPC-cholesterol (3 : 1) mixed bilayer onto a silicon substrate has been investigated in a quantitative manner using atomic force microscopy (AFM) image analysis to extract surface coverage. Complementary AFM-IR measurements were used to confirm the presence of the lipids. For the Langmuir-Blodgett/Schaefer deposition method at temperatures below the chain-melting transition temperature (Tm), a large number of bilayer defects resulted when DMPC was deposited from a water subphase. Addition of calcium ions to the trough led to smaller, more frequent defects, whereas addition of cholesterol to the lipid mixture led to a vast improvement in bilayer coverage. Poor coverage was achieved for deposition at temperatures aboveTm. Formation of the deposited bilayer from vesicle fusion proved a more reliable method for all systems, with formation of near-complete bilayers within 60 seconds at temperatures aboveTm, although this method led to a higher probability of multilayer formation and rougher bilayer surfaces.",
author = "Wood, {Mary H.} and Milan, {David C.} and Nichols, {Richard J.} and Casford, {Michael T.L.} and Horswell, {Sarah L.}",
note = "Funding Information: This work was funded by a Leverhulme Trust Research Project Grant (grant number RPG-2016-158). Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2021.",
year = "2021",
month = jun,
day = "2",
doi = "10.1039/d1ra01920a",
language = "English",
volume = "11",
pages = "19768--19778",
journal = "RSC Advances",
issn = "2046-2069",
publisher = "Royal Society of Chemistry",
number = "32",

}

RIS

TY - JOUR

T1 - A quantitative determination of lipid bilayer deposition efficiency using AFM

AU - Wood, Mary H.

AU - Milan, David C.

AU - Nichols, Richard J.

AU - Casford, Michael T.L.

AU - Horswell, Sarah L.

N1 - Funding Information: This work was funded by a Leverhulme Trust Research Project Grant (grant number RPG-2016-158). Publisher Copyright: © The Royal Society of Chemistry 2021.

PY - 2021/6/2

Y1 - 2021/6/2

N2 - The efficacy of a number of different methods for depositing a dimyristoylphosphatidylcholine (DMPC) lipid bilayer or DMPC-cholesterol (3 : 1) mixed bilayer onto a silicon substrate has been investigated in a quantitative manner using atomic force microscopy (AFM) image analysis to extract surface coverage. Complementary AFM-IR measurements were used to confirm the presence of the lipids. For the Langmuir-Blodgett/Schaefer deposition method at temperatures below the chain-melting transition temperature (Tm), a large number of bilayer defects resulted when DMPC was deposited from a water subphase. Addition of calcium ions to the trough led to smaller, more frequent defects, whereas addition of cholesterol to the lipid mixture led to a vast improvement in bilayer coverage. Poor coverage was achieved for deposition at temperatures aboveTm. Formation of the deposited bilayer from vesicle fusion proved a more reliable method for all systems, with formation of near-complete bilayers within 60 seconds at temperatures aboveTm, although this method led to a higher probability of multilayer formation and rougher bilayer surfaces.

AB - The efficacy of a number of different methods for depositing a dimyristoylphosphatidylcholine (DMPC) lipid bilayer or DMPC-cholesterol (3 : 1) mixed bilayer onto a silicon substrate has been investigated in a quantitative manner using atomic force microscopy (AFM) image analysis to extract surface coverage. Complementary AFM-IR measurements were used to confirm the presence of the lipids. For the Langmuir-Blodgett/Schaefer deposition method at temperatures below the chain-melting transition temperature (Tm), a large number of bilayer defects resulted when DMPC was deposited from a water subphase. Addition of calcium ions to the trough led to smaller, more frequent defects, whereas addition of cholesterol to the lipid mixture led to a vast improvement in bilayer coverage. Poor coverage was achieved for deposition at temperatures aboveTm. Formation of the deposited bilayer from vesicle fusion proved a more reliable method for all systems, with formation of near-complete bilayers within 60 seconds at temperatures aboveTm, although this method led to a higher probability of multilayer formation and rougher bilayer surfaces.

UR - http://www.scopus.com/inward/record.url?scp=85107785077&partnerID=8YFLogxK

U2 - 10.1039/d1ra01920a

DO - 10.1039/d1ra01920a

M3 - Article

AN - SCOPUS:85107785077

VL - 11

SP - 19768

EP - 19778

JO - RSC Advances

JF - RSC Advances

SN - 2046-2069

IS - 32

ER -