TY - JOUR
T1 - A purified plasma membrane fraction isolated from rat liver under isotonic conditions.
AU - Coleman, R
AU - Michell, RH
AU - Finean, JB
AU - Hawthorne, JN
PY - 1967/9
Y1 - 1967/9
N2 - A fraction rich in plasma membrane has been isolated from perfused rat liver. The purity of the preparation is comparable with that of material isolated by other procedures and the yield of the purified fraction is appreciably greater. Isotonic sucrose is used in the early stages of the cell fractionation and it is therefore possible to isolate both a plasma membrane fraction and other cell components from the same homogenate.
AB - A fraction rich in plasma membrane has been isolated from perfused rat liver. The purity of the preparation is comparable with that of material isolated by other procedures and the yield of the purified fraction is appreciably greater. Isotonic sucrose is used in the early stages of the cell fractionation and it is therefore possible to isolate both a plasma membrane fraction and other cell components from the same homogenate.
UR - http://europepmc.org/abstract/med/4292717
U2 - 10.1016/0005-2736(67)90089-2
DO - 10.1016/0005-2736(67)90089-2
M3 - Article
C2 - 4292717
SN - 1874-9399
VL - 135
SP - 573
EP - 579
JO - Biochimica et Biophysica Acta
JF - Biochimica et Biophysica Acta
IS - 4
ER -