A protocol for transverse cardiac slicing and optical mapping in murine heart

Research output: Contribution to journalArticle

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A protocol for transverse cardiac slicing and optical mapping in murine heart. / He, S; Wen, Q; O'Shea, C; Mu-U-Min, R; Kou, K; Grassam-Rowe, A; Liu, Y; Fan, Z; Tan, X; Ou, X; Camelliti, P; Pavlovic, D; Lei, M.

In: Frontiers in Physiology, Vol. 10, 755, 25.06.2019.

Research output: Contribution to journalArticle

Harvard

He, S, Wen, Q, O'Shea, C, Mu-U-Min, R, Kou, K, Grassam-Rowe, A, Liu, Y, Fan, Z, Tan, X, Ou, X, Camelliti, P, Pavlovic, D & Lei, M 2019, 'A protocol for transverse cardiac slicing and optical mapping in murine heart', Frontiers in Physiology, vol. 10, 755. https://doi.org/10.3389/fphys.2019.00755

APA

He, S., Wen, Q., O'Shea, C., Mu-U-Min, R., Kou, K., Grassam-Rowe, A., Liu, Y., Fan, Z., Tan, X., Ou, X., Camelliti, P., Pavlovic, D., & Lei, M. (2019). A protocol for transverse cardiac slicing and optical mapping in murine heart. Frontiers in Physiology, 10, [755]. https://doi.org/10.3389/fphys.2019.00755

Vancouver

Author

He, S ; Wen, Q ; O'Shea, C ; Mu-U-Min, R ; Kou, K ; Grassam-Rowe, A ; Liu, Y ; Fan, Z ; Tan, X ; Ou, X ; Camelliti, P ; Pavlovic, D ; Lei, M. / A protocol for transverse cardiac slicing and optical mapping in murine heart. In: Frontiers in Physiology. 2019 ; Vol. 10.

Bibtex

@article{77ce1e44eb4e426faac2a8c5e6a6abab,
title = "A protocol for transverse cardiac slicing and optical mapping in murine heart",
abstract = "Thin living tissue slices have recently emerged as a new tissue model for cardiac electrophysiological research. Slices can be produced from human cardiac tissue, in addition to small and large mammalian hearts, representing a powerful in vitro model system for preclinical and translational heart research. In the present protocol, we describe a detailed mouse heart transverse slicing and optical imaging methodology. The use of this technology for high-throughput optical imaging allows study of electrophysiology of murine hearts in an organotypic pseudo two-dimensional model. The slices are cut at right angles to the long axis of the heart, permitting robust interrogation of transmembrane potential (Vm) and calcium transients (CaT) throughout the entire heart with exceptional regional precision. This approach enables the use of a series of slices prepared from the ventricles to measure Vm and CaT with high temporal and spatial resolution, allowing (i) comparison of successive slices which form a stack representing the original geometry of the heart; (ii) profiling of transmural and regional gradients in Vm and CaT in the ventricle; (iii) characterization of transmural and regional profiles of action potential and CaT alternans under stress (e.g., high frequency pacing or β-adrenergic stimulation) or pathological conditions (e.g., hypertrophy). Thus, the protocol described here provides a powerful platform for innovative research on electrical and calcium handling heterogeneity within the heart. It can be also combined with optogenetic technology to carry out optical stimulation; aiding studies of cellular Vm and CaT in a cell type specific manner.",
keywords = "optical mapping, transverse cardiac slice, membrane potentials, Ca2+ transients, murine heart",
author = "S He and Q Wen and C O'Shea and R Mu-U-Min and K Kou and A Grassam-Rowe and Y Liu and Z Fan and X Tan and X Ou and P Camelliti and D Pavlovic and M Lei",
year = "2019",
month = jun
day = "25",
doi = "10.3389/fphys.2019.00755",
language = "English",
volume = "10",
journal = "Frontiers in Physiology",
issn = "1664-042X",
publisher = "Frontiers",

}

RIS

TY - JOUR

T1 - A protocol for transverse cardiac slicing and optical mapping in murine heart

AU - He, S

AU - Wen, Q

AU - O'Shea, C

AU - Mu-U-Min, R

AU - Kou, K

AU - Grassam-Rowe, A

AU - Liu, Y

AU - Fan, Z

AU - Tan, X

AU - Ou, X

AU - Camelliti, P

AU - Pavlovic, D

AU - Lei, M

PY - 2019/6/25

Y1 - 2019/6/25

N2 - Thin living tissue slices have recently emerged as a new tissue model for cardiac electrophysiological research. Slices can be produced from human cardiac tissue, in addition to small and large mammalian hearts, representing a powerful in vitro model system for preclinical and translational heart research. In the present protocol, we describe a detailed mouse heart transverse slicing and optical imaging methodology. The use of this technology for high-throughput optical imaging allows study of electrophysiology of murine hearts in an organotypic pseudo two-dimensional model. The slices are cut at right angles to the long axis of the heart, permitting robust interrogation of transmembrane potential (Vm) and calcium transients (CaT) throughout the entire heart with exceptional regional precision. This approach enables the use of a series of slices prepared from the ventricles to measure Vm and CaT with high temporal and spatial resolution, allowing (i) comparison of successive slices which form a stack representing the original geometry of the heart; (ii) profiling of transmural and regional gradients in Vm and CaT in the ventricle; (iii) characterization of transmural and regional profiles of action potential and CaT alternans under stress (e.g., high frequency pacing or β-adrenergic stimulation) or pathological conditions (e.g., hypertrophy). Thus, the protocol described here provides a powerful platform for innovative research on electrical and calcium handling heterogeneity within the heart. It can be also combined with optogenetic technology to carry out optical stimulation; aiding studies of cellular Vm and CaT in a cell type specific manner.

AB - Thin living tissue slices have recently emerged as a new tissue model for cardiac electrophysiological research. Slices can be produced from human cardiac tissue, in addition to small and large mammalian hearts, representing a powerful in vitro model system for preclinical and translational heart research. In the present protocol, we describe a detailed mouse heart transverse slicing and optical imaging methodology. The use of this technology for high-throughput optical imaging allows study of electrophysiology of murine hearts in an organotypic pseudo two-dimensional model. The slices are cut at right angles to the long axis of the heart, permitting robust interrogation of transmembrane potential (Vm) and calcium transients (CaT) throughout the entire heart with exceptional regional precision. This approach enables the use of a series of slices prepared from the ventricles to measure Vm and CaT with high temporal and spatial resolution, allowing (i) comparison of successive slices which form a stack representing the original geometry of the heart; (ii) profiling of transmural and regional gradients in Vm and CaT in the ventricle; (iii) characterization of transmural and regional profiles of action potential and CaT alternans under stress (e.g., high frequency pacing or β-adrenergic stimulation) or pathological conditions (e.g., hypertrophy). Thus, the protocol described here provides a powerful platform for innovative research on electrical and calcium handling heterogeneity within the heart. It can be also combined with optogenetic technology to carry out optical stimulation; aiding studies of cellular Vm and CaT in a cell type specific manner.

KW - optical mapping

KW - transverse cardiac slice

KW - membrane potentials

KW - Ca2+ transients

KW - murine heart

UR - http://www.scopus.com/inward/record.url?scp=85069489497&partnerID=8YFLogxK

U2 - 10.3389/fphys.2019.00755

DO - 10.3389/fphys.2019.00755

M3 - Article

C2 - 31293436

VL - 10

JO - Frontiers in Physiology

JF - Frontiers in Physiology

SN - 1664-042X

M1 - 755

ER -