TY - JOUR
T1 - A protocol for dual calcium-voltage optical mapping in murine sinoatrial preparation with optogenetic pacing
AU - Dong, Ruirui
AU - Mu-U-Min, Razik
AU - Reith, Alastair J M
AU - O'Shea, Christopher
AU - He, Shicheng
AU - Duan, Kaizhong
AU - Kou, Kun
AU - Grassam-Rowe, Alexander
AU - Tan, Xiaoqiu
AU - Pavlovic, Davor
AU - Ou, Xianhong
AU - Lei, Ming
PY - 2019/8/6
Y1 - 2019/8/6
N2 - Among the animal models for studying the molecular basis of atrial and sinoatrial node (SAN) biology and disease, the mouse is a widely used species due to its feasibility for genetic modifications in genes encoding ion channels or calcium handling and signaling proteins in the heart. It is therefore highly valuable to develop robust methodologies for studying SAN and atrial electrophysiological function in this species. Here, we describe a protocol for performing dual calcium-voltage optical mapping on mouse sinoatrial preparation (SAP), in combination with an optogenetic approach, for studying SAP membrane potential, intracellular Ca2+ transients, and pacemaker activity. The protocol includes the details for preparing the intact SAP, robust tissue dual-dye loading, light-programmed pacing, and high-resolution optical mapping. Our protocol provides an example of use of the combination of optogenetic and optical mapping techniques for investigating SAP membrane potential and intracellular Ca2+ transients and pacemaker activity with high temporal and spatial resolution in specific cardiac tissues. Thus, our protocol provides a useful tool for studying SAP physiology and pathophysiology in mice.
AB - Among the animal models for studying the molecular basis of atrial and sinoatrial node (SAN) biology and disease, the mouse is a widely used species due to its feasibility for genetic modifications in genes encoding ion channels or calcium handling and signaling proteins in the heart. It is therefore highly valuable to develop robust methodologies for studying SAN and atrial electrophysiological function in this species. Here, we describe a protocol for performing dual calcium-voltage optical mapping on mouse sinoatrial preparation (SAP), in combination with an optogenetic approach, for studying SAP membrane potential, intracellular Ca2+ transients, and pacemaker activity. The protocol includes the details for preparing the intact SAP, robust tissue dual-dye loading, light-programmed pacing, and high-resolution optical mapping. Our protocol provides an example of use of the combination of optogenetic and optical mapping techniques for investigating SAP membrane potential and intracellular Ca2+ transients and pacemaker activity with high temporal and spatial resolution in specific cardiac tissues. Thus, our protocol provides a useful tool for studying SAP physiology and pathophysiology in mice.
KW - Ca transient
KW - Murine heart
KW - Optical mapping
KW - Optogenetic pacing
KW - Sinoatrial preparation
UR - http://www.scopus.com/inward/record.url?scp=85070571218&partnerID=8YFLogxK
U2 - 10.3389/fphys.2019.00954
DO - 10.3389/fphys.2019.00954
M3 - Article
C2 - 31456689
SN - 1664-042X
VL - 10
JO - Frontiers in Physiology
JF - Frontiers in Physiology
M1 - 954
ER -