A protocol for dual calcium-voltage optical mapping in murine sinoatrial preparation with optogenetic pacing

Research output: Contribution to journalArticle

Authors

  • Ruirui Dong
  • Razik Mu-U-Min
  • Alastair J M Reith
  • Shicheng He
  • Kaizhong Duan
  • Kun Kou
  • Alexander Grassam-Rowe
  • Xiaoqiu Tan
  • Xianhong Ou
  • Ming Lei

Colleges, School and Institutes

External organisations

  • University of Manchester
  • Southwest Medical University

Abstract

Among the animal models for studying the molecular basis of atrial and sinoatrial node (SAN) biology and disease, the mouse is a widely used species due to its feasibility for genetic modifications in genes encoding ion channels or calcium handling and signaling proteins in the heart. It is therefore highly valuable to develop robust methodologies for studying SAN and atrial electrophysiological function in this species. Here, we describe a protocol for performing dual calcium-voltage optical mapping on mouse sinoatrial preparation (SAP), in combination with an optogenetic approach, for studying SAP membrane potential, intracellular Ca2+ transients, and pacemaker activity. The protocol includes the details for preparing the intact SAP, robust tissue dual-dye loading, light-programmed pacing, and high-resolution optical mapping. Our protocol provides an example of use of the combination of optogenetic and optical mapping techniques for investigating SAP membrane potential and intracellular Ca2+ transients and pacemaker activity with high temporal and spatial resolution in specific cardiac tissues. Thus, our protocol provides a useful tool for studying SAP physiology and pathophysiology in mice.

Details

Original languageEnglish
Article number954
JournalFrontiers in Physiology
Volume10
Publication statusPublished - 6 Aug 2019

Keywords

  • Ca transient, Murine heart, Optical mapping, Optogenetic pacing, Sinoatrial preparation

ASJC Scopus subject areas