A potential transcriptional regulator is out-of-frame translated from the metallothionein 2A messenger RNA

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Colleges, School and Institutes


In a number of yeast two-hybrid screens, we have found clones that contained parts of the human metallothionein 2A (MT2A) nucleotide sequence. All of these clones were out-of-frame relative to the MT2A coding sequence and activated the yeast reporters in the presence of the Gal4 DNA binding domain but irrespective of the bait protein. Reporter gene activation was abolished when activation domain and MT2A coding sequences were brought in-frame. In light of these findings, we evaluated all recently reported interactions with metallothioneins because our out-of-frame proline-rich protein might have been the actual interaction partner in some of these studies.


Original languageEnglish
Pages (from-to)159-61
Number of pages3
JournalAnalytical Biochemistry
Issue number1
Publication statusPublished - 1 Feb 2011


  • Base Sequence, DNA, DNA-Binding Proteins, Frameshift Mutation, Genes, Reporter, Humans, Metallothionein, Molecular Sequence Data, Protein Biosynthesis, Protein Structure, Tertiary, RNA, Messenger, Regulatory Elements, Transcriptional, Saccharomyces cerevisiae Proteins, Sequence Alignment, Transcription Factors, Two-Hybrid System Techniques