A novel complex, RUNX1-MYEF2, represses hematopoietic genes in erythroid cells

Research output: Contribution to journalArticle

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A novel complex, RUNX1-MYEF2, represses hematopoietic genes in erythroid cells. / van Riel, Boet; Pakozdi, Tibor; Brouwer, Rutger; Monteiro, Rui; Tuladhar, Kapil; Franke, Vedran; Bryne, Jan Christian; Jorna, Ruud; Rijkers, Erik Jan; van Ijcken, Wilfred; Andrieu-Soler, Charlotte; Demmers, Jeroen; Patient, Roger; Soler, Eric; Lenhard, Boris; Grosveld, Frank.

In: Molecular and Cellular Biology, Vol. 32, No. 19, 01.10.2012, p. 3814-3822.

Research output: Contribution to journalArticle

Harvard

van Riel, B, Pakozdi, T, Brouwer, R, Monteiro, R, Tuladhar, K, Franke, V, Bryne, JC, Jorna, R, Rijkers, EJ, van Ijcken, W, Andrieu-Soler, C, Demmers, J, Patient, R, Soler, E, Lenhard, B & Grosveld, F 2012, 'A novel complex, RUNX1-MYEF2, represses hematopoietic genes in erythroid cells', Molecular and Cellular Biology, vol. 32, no. 19, pp. 3814-3822. https://doi.org/10.1128/MCB.05938-11

APA

van Riel, B., Pakozdi, T., Brouwer, R., Monteiro, R., Tuladhar, K., Franke, V., Bryne, J. C., Jorna, R., Rijkers, E. J., van Ijcken, W., Andrieu-Soler, C., Demmers, J., Patient, R., Soler, E., Lenhard, B., & Grosveld, F. (2012). A novel complex, RUNX1-MYEF2, represses hematopoietic genes in erythroid cells. Molecular and Cellular Biology, 32(19), 3814-3822. https://doi.org/10.1128/MCB.05938-11

Vancouver

Author

van Riel, Boet ; Pakozdi, Tibor ; Brouwer, Rutger ; Monteiro, Rui ; Tuladhar, Kapil ; Franke, Vedran ; Bryne, Jan Christian ; Jorna, Ruud ; Rijkers, Erik Jan ; van Ijcken, Wilfred ; Andrieu-Soler, Charlotte ; Demmers, Jeroen ; Patient, Roger ; Soler, Eric ; Lenhard, Boris ; Grosveld, Frank. / A novel complex, RUNX1-MYEF2, represses hematopoietic genes in erythroid cells. In: Molecular and Cellular Biology. 2012 ; Vol. 32, No. 19. pp. 3814-3822.

Bibtex

@article{a0475f863feb4cb9b88d0834a97df15a,
title = "A novel complex, RUNX1-MYEF2, represses hematopoietic genes in erythroid cells",
abstract = "RUNX1 is known to be an essential transcription factor for generating hematopoietic stem cells (HSC), but much less is known about its role in the downstream process of hematopoietic differentiation. RUNX1 has been shown to be part of a large transcription factor complex, together with LDB1, GATA1, TAL1, and ETO2 (N. Meier et al., Development 133:4913- 4923, 2006) in erythroid cells. We used a tagging strategy to show that RUNX1 interacts with two novel protein partners, LSD1 and MYEF2, in erythroid cells. MYEF2 is bound in undifferentiated cells and is lost upon differentiation, whereas LSD1 is bound in differentiated cells. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) and microarray expression analysis were used to show that RUNX1 binds approximately 9,000 target sites in erythroid cells and is primarily active in the undifferentiated state. Functional analysis shows that a subset of the target genes is suppressed by RUNX1 via the newly identified partner MYEF2. Knockdown of Myef2 expression in developing zebrafish results in a reduced number of HSC.",
author = "{van Riel}, Boet and Tibor Pakozdi and Rutger Brouwer and Rui Monteiro and Kapil Tuladhar and Vedran Franke and Bryne, {Jan Christian} and Ruud Jorna and Rijkers, {Erik Jan} and {van Ijcken}, Wilfred and Charlotte Andrieu-Soler and Jeroen Demmers and Roger Patient and Eric Soler and Boris Lenhard and Frank Grosveld",
year = "2012",
month = oct,
day = "1",
doi = "10.1128/MCB.05938-11",
language = "English",
volume = "32",
pages = "3814--3822",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "19",

}

RIS

TY - JOUR

T1 - A novel complex, RUNX1-MYEF2, represses hematopoietic genes in erythroid cells

AU - van Riel, Boet

AU - Pakozdi, Tibor

AU - Brouwer, Rutger

AU - Monteiro, Rui

AU - Tuladhar, Kapil

AU - Franke, Vedran

AU - Bryne, Jan Christian

AU - Jorna, Ruud

AU - Rijkers, Erik Jan

AU - van Ijcken, Wilfred

AU - Andrieu-Soler, Charlotte

AU - Demmers, Jeroen

AU - Patient, Roger

AU - Soler, Eric

AU - Lenhard, Boris

AU - Grosveld, Frank

PY - 2012/10/1

Y1 - 2012/10/1

N2 - RUNX1 is known to be an essential transcription factor for generating hematopoietic stem cells (HSC), but much less is known about its role in the downstream process of hematopoietic differentiation. RUNX1 has been shown to be part of a large transcription factor complex, together with LDB1, GATA1, TAL1, and ETO2 (N. Meier et al., Development 133:4913- 4923, 2006) in erythroid cells. We used a tagging strategy to show that RUNX1 interacts with two novel protein partners, LSD1 and MYEF2, in erythroid cells. MYEF2 is bound in undifferentiated cells and is lost upon differentiation, whereas LSD1 is bound in differentiated cells. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) and microarray expression analysis were used to show that RUNX1 binds approximately 9,000 target sites in erythroid cells and is primarily active in the undifferentiated state. Functional analysis shows that a subset of the target genes is suppressed by RUNX1 via the newly identified partner MYEF2. Knockdown of Myef2 expression in developing zebrafish results in a reduced number of HSC.

AB - RUNX1 is known to be an essential transcription factor for generating hematopoietic stem cells (HSC), but much less is known about its role in the downstream process of hematopoietic differentiation. RUNX1 has been shown to be part of a large transcription factor complex, together with LDB1, GATA1, TAL1, and ETO2 (N. Meier et al., Development 133:4913- 4923, 2006) in erythroid cells. We used a tagging strategy to show that RUNX1 interacts with two novel protein partners, LSD1 and MYEF2, in erythroid cells. MYEF2 is bound in undifferentiated cells and is lost upon differentiation, whereas LSD1 is bound in differentiated cells. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) and microarray expression analysis were used to show that RUNX1 binds approximately 9,000 target sites in erythroid cells and is primarily active in the undifferentiated state. Functional analysis shows that a subset of the target genes is suppressed by RUNX1 via the newly identified partner MYEF2. Knockdown of Myef2 expression in developing zebrafish results in a reduced number of HSC.

UR - http://www.scopus.com/inward/record.url?scp=84868697416&partnerID=8YFLogxK

U2 - 10.1128/MCB.05938-11

DO - 10.1128/MCB.05938-11

M3 - Article

C2 - 22801375

AN - SCOPUS:84868697416

VL - 32

SP - 3814

EP - 3822

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 19

ER -