A novel cell surface marker expressed on human embryonic hepatoblasts and a subpopulation of hepatic biliary cells

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A novel cell surface marker expressed on human embryonic hepatoblasts and a subpopulation of hepatic biliary cells. / Stamp, L; Crosby, Heather; Hawes, S; Strain, Alastair; Pera, MF.

In: Stem Cells, Vol. 23, No. 1, 01.01.2005, p. 103-112.

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@article{4069be4b108144639f01fc3365d749a9,
title = "A novel cell surface marker expressed on human embryonic hepatoblasts and a subpopulation of hepatic biliary cells",
abstract = "The nature of the cells that contribute to the repopulation of the liver after hepatic necrosis or cirrhosis remains uncertain, in part because we lack specific markers to facilitate identification and prospective isolation of progenitor cells. The monoclonal antibody GCTM-5 reacts with a minority subpopulation of cells in spontaneously differentiating cultures of pluripotent human embryonal carcinoma or embryonic stem cells. The epitope recognized by GCTM-5 is found on a 50-kDa protein present on the surface of these cells. In tissue sections of first-trimester human embryos, GCTM-5 specifically stained hepatoblasts and no other cell type examined. In normal pediatric or adult liver, GCTM-5 reacted with a minority population of luminal bile duct cells. In diseased livers, the numbers of GCTM-5-positive cells were increased compared with normal liver; antibody staining was restricted to a subpopulation of ductular reactive cells, and among this subpopulation we observed GCTM-5-positive cells that did not express cytokeratin 19 or N-CAM, classical makers of ductular reactive cells. Live GCTM-5-positive cells could be isolated from diseased livers by immunomagnetic sorting. These results suggest that GCTM-5 will be a useful reagent for defining cell lineage relationships between putative progenitor populations in embryonic liver and in the biliary epithelium during tissue repair.",
keywords = "endoderm, liver stem cell, differentiation, embryonic stem cell, monoclonal antibody GCTM-5",
author = "L Stamp and Heather Crosby and S Hawes and Alastair Strain and MF Pera",
year = "2005",
month = jan,
day = "1",
doi = "10.1634/stemcells.2004-0147",
language = "English",
volume = "23",
pages = "103--112",
journal = "Stem Cells",
issn = "1066-5099",
publisher = "AlphaMed Press",
number = "1",

}

RIS

TY - JOUR

T1 - A novel cell surface marker expressed on human embryonic hepatoblasts and a subpopulation of hepatic biliary cells

AU - Stamp, L

AU - Crosby, Heather

AU - Hawes, S

AU - Strain, Alastair

AU - Pera, MF

PY - 2005/1/1

Y1 - 2005/1/1

N2 - The nature of the cells that contribute to the repopulation of the liver after hepatic necrosis or cirrhosis remains uncertain, in part because we lack specific markers to facilitate identification and prospective isolation of progenitor cells. The monoclonal antibody GCTM-5 reacts with a minority subpopulation of cells in spontaneously differentiating cultures of pluripotent human embryonal carcinoma or embryonic stem cells. The epitope recognized by GCTM-5 is found on a 50-kDa protein present on the surface of these cells. In tissue sections of first-trimester human embryos, GCTM-5 specifically stained hepatoblasts and no other cell type examined. In normal pediatric or adult liver, GCTM-5 reacted with a minority population of luminal bile duct cells. In diseased livers, the numbers of GCTM-5-positive cells were increased compared with normal liver; antibody staining was restricted to a subpopulation of ductular reactive cells, and among this subpopulation we observed GCTM-5-positive cells that did not express cytokeratin 19 or N-CAM, classical makers of ductular reactive cells. Live GCTM-5-positive cells could be isolated from diseased livers by immunomagnetic sorting. These results suggest that GCTM-5 will be a useful reagent for defining cell lineage relationships between putative progenitor populations in embryonic liver and in the biliary epithelium during tissue repair.

AB - The nature of the cells that contribute to the repopulation of the liver after hepatic necrosis or cirrhosis remains uncertain, in part because we lack specific markers to facilitate identification and prospective isolation of progenitor cells. The monoclonal antibody GCTM-5 reacts with a minority subpopulation of cells in spontaneously differentiating cultures of pluripotent human embryonal carcinoma or embryonic stem cells. The epitope recognized by GCTM-5 is found on a 50-kDa protein present on the surface of these cells. In tissue sections of first-trimester human embryos, GCTM-5 specifically stained hepatoblasts and no other cell type examined. In normal pediatric or adult liver, GCTM-5 reacted with a minority population of luminal bile duct cells. In diseased livers, the numbers of GCTM-5-positive cells were increased compared with normal liver; antibody staining was restricted to a subpopulation of ductular reactive cells, and among this subpopulation we observed GCTM-5-positive cells that did not express cytokeratin 19 or N-CAM, classical makers of ductular reactive cells. Live GCTM-5-positive cells could be isolated from diseased livers by immunomagnetic sorting. These results suggest that GCTM-5 will be a useful reagent for defining cell lineage relationships between putative progenitor populations in embryonic liver and in the biliary epithelium during tissue repair.

KW - endoderm

KW - liver stem cell

KW - differentiation

KW - embryonic stem cell

KW - monoclonal antibody GCTM-5

UR - http://www.scopus.com/inward/record.url?scp=12144279646&partnerID=8YFLogxK

U2 - 10.1634/stemcells.2004-0147

DO - 10.1634/stemcells.2004-0147

M3 - Article

C2 - 15625127

VL - 23

SP - 103

EP - 112

JO - Stem Cells

JF - Stem Cells

SN - 1066-5099

IS - 1

ER -