A non-canonical promoter element drives spurious transcription of horizontally acquired bacterial genes

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A non-canonical promoter element drives spurious transcription of horizontally acquired bacterial genes. / Warman, Emily A; Singh, Shivani S; Gubieda, Alicia G; Grainger, David C.

In: Nucleic Acids Research, Vol. 48, No. 9, 21.05.2020, p. 4891-4901.

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Warman, Emily A ; Singh, Shivani S ; Gubieda, Alicia G ; Grainger, David C. / A non-canonical promoter element drives spurious transcription of horizontally acquired bacterial genes. In: Nucleic Acids Research. 2020 ; Vol. 48, No. 9. pp. 4891-4901.

Bibtex

@article{5a3623055f2d4b1fb5826da201d6cb50,
title = "A non-canonical promoter element drives spurious transcription of horizontally acquired bacterial genes",
abstract = "RNA polymerases initiate transcription at DNA sequences called promoters. In bacteria, the best conserved promoter feature is the AT-rich -10 element; a sequence essential for DNA unwinding. Further elements, and gene regulatory proteins, are needed to recruit RNA polymerase to the -10 sequence. Hence, -10 elements cannot function in isolation. Many horizontally acquired genes also have a high AT-content. Consequently, sequences that resemble the -10 element occur frequently. As a result, foreign genes are predisposed to spurious transcription. However, it is not clear how RNA polymerase initially recognizes such sequences. Here, we identify a non-canonical promoter element that plays a key role. The sequence, itself a short AT-tract, resides 5 base pairs upstream of otherwise cryptic -10 elements. The AT-tract alters DNA conformation and enhances contacts between the DNA backbone and RNA polymerase.",
author = "Warman, {Emily A} and Singh, {Shivani S} and Gubieda, {Alicia G} and Grainger, {David C}",
note = "{\textcopyright} The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.",
year = "2020",
month = may,
day = "21",
doi = "10.1093/nar/gkaa244",
language = "English",
volume = "48",
pages = "4891--4901",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "9",

}

RIS

TY - JOUR

T1 - A non-canonical promoter element drives spurious transcription of horizontally acquired bacterial genes

AU - Warman, Emily A

AU - Singh, Shivani S

AU - Gubieda, Alicia G

AU - Grainger, David C

N1 - © The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

PY - 2020/5/21

Y1 - 2020/5/21

N2 - RNA polymerases initiate transcription at DNA sequences called promoters. In bacteria, the best conserved promoter feature is the AT-rich -10 element; a sequence essential for DNA unwinding. Further elements, and gene regulatory proteins, are needed to recruit RNA polymerase to the -10 sequence. Hence, -10 elements cannot function in isolation. Many horizontally acquired genes also have a high AT-content. Consequently, sequences that resemble the -10 element occur frequently. As a result, foreign genes are predisposed to spurious transcription. However, it is not clear how RNA polymerase initially recognizes such sequences. Here, we identify a non-canonical promoter element that plays a key role. The sequence, itself a short AT-tract, resides 5 base pairs upstream of otherwise cryptic -10 elements. The AT-tract alters DNA conformation and enhances contacts between the DNA backbone and RNA polymerase.

AB - RNA polymerases initiate transcription at DNA sequences called promoters. In bacteria, the best conserved promoter feature is the AT-rich -10 element; a sequence essential for DNA unwinding. Further elements, and gene regulatory proteins, are needed to recruit RNA polymerase to the -10 sequence. Hence, -10 elements cannot function in isolation. Many horizontally acquired genes also have a high AT-content. Consequently, sequences that resemble the -10 element occur frequently. As a result, foreign genes are predisposed to spurious transcription. However, it is not clear how RNA polymerase initially recognizes such sequences. Here, we identify a non-canonical promoter element that plays a key role. The sequence, itself a short AT-tract, resides 5 base pairs upstream of otherwise cryptic -10 elements. The AT-tract alters DNA conformation and enhances contacts between the DNA backbone and RNA polymerase.

U2 - 10.1093/nar/gkaa244

DO - 10.1093/nar/gkaa244

M3 - Article

C2 - 32297955

VL - 48

SP - 4891

EP - 4901

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 9

ER -