A new minimal deleted region at 11q22.3 reveals the importance of interpretation of diminished FISH signals and the choice of probe for ATM deletion screening in chronic lymphocytic leukemia

A Gardiner; H Parker; S Glide; S Mould; H Robinson; I Tracy; Tatjana Stankovic; D Oscier; J Strefford

doi:10.1016/j.leukres.2011.08.002

A new minimal deleted region at 11q22.3 reveals the importance of interpretation of diminished FISH signals and the choice of probe for ATM deletion screening in chronic lymphocytic leukemia

A Gardiner, H Parker, S Glide, S Mould, H Robinson, I Tracy, Tatjana Stankovic, D Oscier, J Strefford

Cancer and Genomic Sciences

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7 Citations (Scopus)

Abstract

Deletion of ATM detected by fluorescent in situ hybridization (FISH) in chronic lymphocytic leukemia predicts short treatment free survival and poor outcome following alkylator/purine analogue therapy. We describe five cases, with a diminished ATM FISH signal, investigated by TP53 mutation/dysfunction studies and single nucleotide polymorphism (SNP) array. The diminished signal represented loss of the ATM gene, which could have been missed were the cases not further investigated. These rare cases highlight the need for careful consideration of the choice of probe and interpretation of unusual signal patterns in FISH screening. We define a new minimal region of deletion at 11q22.3. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	307-310
Number of pages	4
Journal	Leukemia Research
Volume	36
Issue number	3
DOIs	https://doi.org/10.1016/j.leukres.2011.08.002
Publication status	Published - 1 Mar 2012

Keywords

11q23 MDR
Diminished FISH signal
CLL
FISH
SNP array
ATM screening

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10.1016/j.leukres.2011.08.002

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Gardiner, A., Parker, H., Glide, S., Mould, S., Robinson, H., Tracy, I., Stankovic, T., Oscier, D., & Strefford, J. (2012). A new minimal deleted region at 11q22.3 reveals the importance of interpretation of diminished FISH signals and the choice of probe for ATM deletion screening in chronic lymphocytic leukemia. Leukemia Research, 36(3), 307-310. https://doi.org/10.1016/j.leukres.2011.08.002

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title = "A new minimal deleted region at 11q22.3 reveals the importance of interpretation of diminished FISH signals and the choice of probe for ATM deletion screening in chronic lymphocytic leukemia",

abstract = "Deletion of ATM detected by fluorescent in situ hybridization (FISH) in chronic lymphocytic leukemia predicts short treatment free survival and poor outcome following alkylator/purine analogue therapy. We describe five cases, with a diminished ATM FISH signal, investigated by TP53 mutation/dysfunction studies and single nucleotide polymorphism (SNP) array. The diminished signal represented loss of the ATM gene, which could have been missed were the cases not further investigated. These rare cases highlight the need for careful consideration of the choice of probe and interpretation of unusual signal patterns in FISH screening. We define a new minimal region of deletion at 11q22.3. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.",

keywords = "11q23 MDR, Diminished FISH signal, CLL, FISH, SNP array, ATM screening",

author = "A Gardiner and H Parker and S Glide and S Mould and H Robinson and I Tracy and Tatjana Stankovic and D Oscier and J Strefford",

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Gardiner, A, Parker, H, Glide, S, Mould, S, Robinson, H, Tracy, I, Stankovic, T, Oscier, D & Strefford, J 2012, 'A new minimal deleted region at 11q22.3 reveals the importance of interpretation of diminished FISH signals and the choice of probe for ATM deletion screening in chronic lymphocytic leukemia', Leukemia Research, vol. 36, no. 3, pp. 307-310. https://doi.org/10.1016/j.leukres.2011.08.002

TY - JOUR

T1 - A new minimal deleted region at 11q22.3 reveals the importance of interpretation of diminished FISH signals and the choice of probe for ATM deletion screening in chronic lymphocytic leukemia

AU - Gardiner, A

AU - Parker, H

AU - Glide, S

AU - Mould, S

AU - Robinson, H

AU - Tracy, I

AU - Stankovic, Tatjana

AU - Oscier, D

AU - Strefford, J

PY - 2012/3/1

Y1 - 2012/3/1

N2 - Deletion of ATM detected by fluorescent in situ hybridization (FISH) in chronic lymphocytic leukemia predicts short treatment free survival and poor outcome following alkylator/purine analogue therapy. We describe five cases, with a diminished ATM FISH signal, investigated by TP53 mutation/dysfunction studies and single nucleotide polymorphism (SNP) array. The diminished signal represented loss of the ATM gene, which could have been missed were the cases not further investigated. These rare cases highlight the need for careful consideration of the choice of probe and interpretation of unusual signal patterns in FISH screening. We define a new minimal region of deletion at 11q22.3. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.

AB - Deletion of ATM detected by fluorescent in situ hybridization (FISH) in chronic lymphocytic leukemia predicts short treatment free survival and poor outcome following alkylator/purine analogue therapy. We describe five cases, with a diminished ATM FISH signal, investigated by TP53 mutation/dysfunction studies and single nucleotide polymorphism (SNP) array. The diminished signal represented loss of the ATM gene, which could have been missed were the cases not further investigated. These rare cases highlight the need for careful consideration of the choice of probe and interpretation of unusual signal patterns in FISH screening. We define a new minimal region of deletion at 11q22.3. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.

KW - 11q23 MDR

KW - Diminished FISH signal

KW - CLL

KW - FISH

KW - SNP array

KW - ATM screening

U2 - 10.1016/j.leukres.2011.08.002

DO - 10.1016/j.leukres.2011.08.002

M3 - Article

C2 - 21955805

SN - 1873-5835

VL - 36

SP - 307

EP - 310

JO - Leukemia Research

JF - Leukemia Research

IS - 3

ER -

A new minimal deleted region at 11q22.3 reveals the importance of interpretation of diminished FISH signals and the choice of probe for ATM deletion screening in chronic lymphocytic leukemia

Abstract

Keywords

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