TY - JOUR
T1 - A methodology to establish a database to study gene environment interactions for childhood asthma
AU - Turner, SW
AU - Ayres, Jonathan
AU - Macfarlane, TV
AU - Mehta, A
AU - Mehta, G
AU - Palmer, CN
AU - Cunningham, S
AU - Adams, T
AU - Aniruddhan, K
AU - Bell, C
AU - Corrigan, D
AU - Cunningham, J
AU - Duncan, A
AU - Hunt, G
AU - Leece, R
AU - MacFadyen, U
AU - McCormick, J
AU - McLeish, S
AU - Mitra, A
AU - Miller, D
AU - Waxman, E
AU - Webb, A
AU - Wojcik, S
AU - Mukhopadhyay, S
AU - Macgregor, D
PY - 2010/12/6
Y1 - 2010/12/6
N2 - Background: Gene-environment interactions are likely to explain some of the heterogeneity in childhood asthma. Here, we describe the methodology and experiences in establishing a database for childhood asthma designed to study gene-environment interactions (PAGES - Paediatric Asthma Gene Environment Study).
Methods: Children with asthma and under the care of a respiratory paediatrician are being recruited from 15 hospitals between 2008 and 2011. An asthma questionnaire is completed and returned by post. At a routine clinic visit saliva is collected for DNA extraction. Detailed phenotyping in a proportion of children includes spirometry, bronchodilator response (BDR), skin prick reactivity, exhaled nitric oxide and salivary cotinine. Dietary and quality of life questionnaires are completed. Data are entered onto a purpose-built database.
Results: To date 1045 children have been invited to participate and data collected in 501 (48%). The mean age (SD) of participants is 8.6 (3.9) years, 57% male. DNA has been collected in 436 children. Spirometry has been obtained in 172 children, mean % predicted (SD) FEV1 97% (15) and median (IQR) BDR is 5% (2, 9). There were differences in age, socioeconomic status, severity and % FEV1 between the different centres (p
AB - Background: Gene-environment interactions are likely to explain some of the heterogeneity in childhood asthma. Here, we describe the methodology and experiences in establishing a database for childhood asthma designed to study gene-environment interactions (PAGES - Paediatric Asthma Gene Environment Study).
Methods: Children with asthma and under the care of a respiratory paediatrician are being recruited from 15 hospitals between 2008 and 2011. An asthma questionnaire is completed and returned by post. At a routine clinic visit saliva is collected for DNA extraction. Detailed phenotyping in a proportion of children includes spirometry, bronchodilator response (BDR), skin prick reactivity, exhaled nitric oxide and salivary cotinine. Dietary and quality of life questionnaires are completed. Data are entered onto a purpose-built database.
Results: To date 1045 children have been invited to participate and data collected in 501 (48%). The mean age (SD) of participants is 8.6 (3.9) years, 57% male. DNA has been collected in 436 children. Spirometry has been obtained in 172 children, mean % predicted (SD) FEV1 97% (15) and median (IQR) BDR is 5% (2, 9). There were differences in age, socioeconomic status, severity and % FEV1 between the different centres (p
U2 - 10.1186/1471-2288-10-107
DO - 10.1186/1471-2288-10-107
M3 - Article
C2 - 21134251
SN - 1471-2288
VL - 10
JO - BMC Medical Research Methodology
JF - BMC Medical Research Methodology
M1 - 107
ER -