A methodology to establish a database to study gene environment interactions for childhood asthma

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  • SW Turner
  • TV Macfarlane
  • A Mehta
  • G Mehta
  • CN Palmer
  • S Cunningham
  • T Adams
  • K Aniruddhan
  • C Bell
  • D Corrigan
  • J Cunningham
  • A Duncan
  • G Hunt
  • R Leece
  • U MacFadyen
  • J McCormick
  • S McLeish
  • A Mitra
  • D Miller
  • E Waxman
  • A Webb
  • S Wojcik
  • S Mukhopadhyay
  • D Macgregor


Background: Gene-environment interactions are likely to explain some of the heterogeneity in childhood asthma. Here, we describe the methodology and experiences in establishing a database for childhood asthma designed to study gene-environment interactions (PAGES - Paediatric Asthma Gene Environment Study). Methods: Children with asthma and under the care of a respiratory paediatrician are being recruited from 15 hospitals between 2008 and 2011. An asthma questionnaire is completed and returned by post. At a routine clinic visit saliva is collected for DNA extraction. Detailed phenotyping in a proportion of children includes spirometry, bronchodilator response (BDR), skin prick reactivity, exhaled nitric oxide and salivary cotinine. Dietary and quality of life questionnaires are completed. Data are entered onto a purpose-built database. Results: To date 1045 children have been invited to participate and data collected in 501 (48%). The mean age (SD) of participants is 8.6 (3.9) years, 57% male. DNA has been collected in 436 children. Spirometry has been obtained in 172 children, mean % predicted (SD) FEV1 97% (15) and median (IQR) BDR is 5% (2, 9). There were differences in age, socioeconomic status, severity and % FEV1 between the different centres (p


Original languageEnglish
Article number107
JournalBMC Medical Research Methodology
Publication statusPublished - 6 Dec 2010