A method for generating marker-less gene deletions in multidrug-resistant Acinetobacter baumannii

Ismawati Mohammad Amin; Grace E Richmond; Paromita Sen; Tse Hsien Koh; Laura J V Piddock; Kim Lee Chua

doi:10.1186/1471-2180-13-158

A method for generating marker-less gene deletions in multidrug-resistant Acinetobacter baumannii

Ismawati Mohammad Amin, Grace E Richmond, Paromita Sen, Tse Hsien Koh, Laura J V Piddock, Kim Lee Chua

Immunology and Immunotherapy

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Abstract

Acinetobacter baumannii is an important nosocomial pathogen that has become increasingly resistant to multiple antibiotics. Genetic manipulation of MDR A. baumannii is useful especially for defining the contribution of each active efflux mechanism in multidrug resistance. Existing methods rely on the use of an antibiotic selection marker and are not suited for multiple gene deletions.

Original language	English
Article number	158
Journal	BMC Microbiology
Volume	13
DOIs	https://doi.org/10.1186/1471-2180-13-158
Publication status	Published - 13 Jul 2013

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10.1186/1471-2180-13-158

Amin_et_al_Method_for_generating_marker-less_gene_deletions_BMC_Microbiology_2013
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Checked July 2015
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title = "A method for generating marker-less gene deletions in multidrug-resistant Acinetobacter baumannii",

abstract = "Acinetobacter baumannii is an important nosocomial pathogen that has become increasingly resistant to multiple antibiotics. Genetic manipulation of MDR A. baumannii is useful especially for defining the contribution of each active efflux mechanism in multidrug resistance. Existing methods rely on the use of an antibiotic selection marker and are not suited for multiple gene deletions.",

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AU - Sen, Paromita

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AU - Piddock, Laura J V

AU - Chua, Kim Lee

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AB - Acinetobacter baumannii is an important nosocomial pathogen that has become increasingly resistant to multiple antibiotics. Genetic manipulation of MDR A. baumannii is useful especially for defining the contribution of each active efflux mechanism in multidrug resistance. Existing methods rely on the use of an antibiotic selection marker and are not suited for multiple gene deletions.

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DO - 10.1186/1471-2180-13-158

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JO - BMC Microbiology

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A method for generating marker-less gene deletions in multidrug-resistant Acinetobacter baumannii

Abstract

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