A mesocosm study of fate and effects of CuO nanoparticles on endobenthic species (Scrobicularia plana, Hediste diversicolor)

Research output: Contribution to journalArticle

Authors

  • Pierre-Emmanuel Buffet
  • Marion Richard
  • Fanny Caupos
  • Aurore Vergnoux
  • Hanane Perrein-Ettajani
  • Andrea Luna-Acosta
  • Farida Akcha
  • Jean-Claude Amiard
  • Claude Amiard-Triquet
  • Marielle Guibbolini
  • Christine Risso-De Faverney
  • Helene Thomas-Guyon
  • Paul Reip
  • Agnieska Dybowska
  • Deborah Berhanu
  • Catherine Mouneyrac

Colleges, School and Institutes

Abstract

The fate and effects of CuO nanoparticles (CuO NPs) were examined in endobenthic species (Scrobicularia plana , Hediste diversicolor), under environmentally realistic conditions in outdoor mesocosms (exposure to Cu at 10 μg L(-1) in particulate (CuO NPs) or soluble salt (CuNO(3)) forms) for 21 days. Labile Cu was determined in water and sediment by using diffusive gradient in thin films. No labile Cu being detected from CuO NPs; the observed effects in invertebrates exposed to CuO NPs were mainly attributed to the toxicity of nanoparticulate rather than dissolved Cu toxicity. Bioaccumulation of CuO NPs was observed in both species. Biomarkers were examined at different levels of biological organization: biochemical markers of defense and damage, biomarkers of genotoxicity (comet assay), and behavioral biomarkers (feeding and burrowing). Behavioral biomarkers, antioxidant defenses (catalase, glutathion S-transferase, metallothionein), and genotoxicity are the most sensitive tools to highlight the effect of soluble or nanoparticulate metal forms. Concerning other biomarkers of defense (superoxide dismutase, lactate dehydrogenase, laccase) and damage (thiobarbituric acid reactive substances, acetylcholinesterase, acid phosphatase), no significant effects were detected. This experiment shows the suitability of mesocosms for studying the environmental effects of nanoparticles.

Details

Original languageEnglish
Pages (from-to)1620-8
Number of pages9
JournalEnvironmental Science & Technology
Volume47
Issue number3
Publication statusPublished - 5 Feb 2013

Keywords

  • Animals, Behavior, Animal, Biological Markers, Bivalvia, Comet Assay, Copper, DNA Damage, Ecosystem, Environmental Monitoring, France, Metal Nanoparticles, Polychaeta