TY - JOUR
T1 - A mesocosm study of fate and effects of CuO nanoparticles on endobenthic species (Scrobicularia plana, Hediste diversicolor)
AU - Buffet, Pierre-Emmanuel
AU - Richard, Marion
AU - Caupos, Fanny
AU - Vergnoux, Aurore
AU - Perrein-Ettajani, Hanane
AU - Luna-Acosta, Andrea
AU - Akcha, Farida
AU - Amiard, Jean-Claude
AU - Amiard-Triquet, Claude
AU - Guibbolini, Marielle
AU - Risso-De Faverney, Christine
AU - Thomas-Guyon, Helene
AU - Reip, Paul
AU - Dybowska, Agnieska
AU - Berhanu, Deborah
AU - Valsami-Jones, Eugenia
AU - Mouneyrac, Catherine
PY - 2013/2/5
Y1 - 2013/2/5
N2 - The fate and effects of CuO nanoparticles (CuO NPs) were examined in endobenthic species (Scrobicularia plana , Hediste diversicolor), under environmentally realistic conditions in outdoor mesocosms (exposure to Cu at 10 μg L(-1) in particulate (CuO NPs) or soluble salt (CuNO(3)) forms) for 21 days. Labile Cu was determined in water and sediment by using diffusive gradient in thin films. No labile Cu being detected from CuO NPs; the observed effects in invertebrates exposed to CuO NPs were mainly attributed to the toxicity of nanoparticulate rather than dissolved Cu toxicity. Bioaccumulation of CuO NPs was observed in both species. Biomarkers were examined at different levels of biological organization: biochemical markers of defense and damage, biomarkers of genotoxicity (comet assay), and behavioral biomarkers (feeding and burrowing). Behavioral biomarkers, antioxidant defenses (catalase, glutathion S-transferase, metallothionein), and genotoxicity are the most sensitive tools to highlight the effect of soluble or nanoparticulate metal forms. Concerning other biomarkers of defense (superoxide dismutase, lactate dehydrogenase, laccase) and damage (thiobarbituric acid reactive substances, acetylcholinesterase, acid phosphatase), no significant effects were detected. This experiment shows the suitability of mesocosms for studying the environmental effects of nanoparticles.
AB - The fate and effects of CuO nanoparticles (CuO NPs) were examined in endobenthic species (Scrobicularia plana , Hediste diversicolor), under environmentally realistic conditions in outdoor mesocosms (exposure to Cu at 10 μg L(-1) in particulate (CuO NPs) or soluble salt (CuNO(3)) forms) for 21 days. Labile Cu was determined in water and sediment by using diffusive gradient in thin films. No labile Cu being detected from CuO NPs; the observed effects in invertebrates exposed to CuO NPs were mainly attributed to the toxicity of nanoparticulate rather than dissolved Cu toxicity. Bioaccumulation of CuO NPs was observed in both species. Biomarkers were examined at different levels of biological organization: biochemical markers of defense and damage, biomarkers of genotoxicity (comet assay), and behavioral biomarkers (feeding and burrowing). Behavioral biomarkers, antioxidant defenses (catalase, glutathion S-transferase, metallothionein), and genotoxicity are the most sensitive tools to highlight the effect of soluble or nanoparticulate metal forms. Concerning other biomarkers of defense (superoxide dismutase, lactate dehydrogenase, laccase) and damage (thiobarbituric acid reactive substances, acetylcholinesterase, acid phosphatase), no significant effects were detected. This experiment shows the suitability of mesocosms for studying the environmental effects of nanoparticles.
KW - Animals
KW - Behavior, Animal
KW - Biological Markers
KW - Bivalvia
KW - Comet Assay
KW - Copper
KW - DNA Damage
KW - Ecosystem
KW - Environmental Monitoring
KW - France
KW - Metal Nanoparticles
KW - Polychaeta
U2 - 10.1021/es303513r
DO - 10.1021/es303513r
M3 - Article
C2 - 23240726
SN - 0013-936X
VL - 47
SP - 1620
EP - 1628
JO - Environmental Science & Technology
JF - Environmental Science & Technology
IS - 3
ER -