A high throughput imaging database of toxicological effects of nanomaterials tested on HepaRG cells

Research output: Contribution to journalArticle

Authors

  • Elisabeth Joossens
  • Peter Macko
  • Taina Palosaari
  • Kirsten Gerloff
  • Isaac Ojea-Jiménez
  • Douglas Gilliland
  • Jaroslav Novak
  • Salvador Fortaner Torrent
  • Jean-Michel Gineste
  • Isabella Römer
  • Maurice Whelan

Colleges, School and Institutes

External organisations

  • European Commission Joint Research Centre (JRC), Ispra (VA), Italy
  • Human & Environmental Health & Safety Group, Materials Safety Unit, LEITAT, C/Palllars 179-185, 08005, Barcelona, Spain.
  • School of Geography Earth, and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
  • European Commission, Joint Research Centre (JRC), Ispra, Italy. maurice.whelan@ec.europa.eu.

Abstract

The large amount of existing nanomaterials demands rapid and reliable methods for testing their potential toxicological effect on human health, preferably by means of relevant in vitro techniques in order to reduce testing on animals. Combining high throughput workflows with automated high content imaging techniques allows deriving much more information from cell-based assays than the typical readouts (i.e. one measurement per well) with optical plate-readers. We present here a dataset including data based on a maximum of 14 different read outs (including viable cell count, cell membrane permeability, apoptotic cell death, mitochondrial membrane potential and steatosis) of the human hepatoma HepaRG cell line treated with a large set of nanomaterials, coatings and supernatants at different concentrations. The database, given its size, can be utilized in the development of in silico hazard assessment and prediction tools or can be combined with toxicity results from other in vitro test systems.

Details

Original languageEnglish
Article number46
Number of pages10
JournalScientific Data
Volume6
Issue number1
Publication statusPublished - 2 May 2019

Keywords

  • Apoptosis/drug effects, Carcinoma, Hepatocellular/pathology, Cell Count, Cell Line, Tumor, Cell Membrane Permeability/drug effects, Cell Survival/drug effects, Databases, Factual, Dose-Response Relationship, Drug, Humans, Liver Neoplasms/pathology, Membrane Potential, Mitochondrial/drug effects, Nanostructures/toxicity