A culture-independent sequence-based metagenomics approach to the investigation of an outbreak of Shiga-toxigenic Escherichia coli O104:H4

Research output: Contribution to journalArticlepeer-review


  • Martin Christner
  • Holger Rohde
  • Jacqueline C Weir
  • Christopher Quince
  • Geoffrey P Smith
  • Jason R Betley
  • Martin Aepfelbacher

Colleges, School and Institutes


Identification of the bacterium responsible for an outbreak can aid in disease management. However, traditional culture-based diagnosis can be difficult, particularly if no specific diagnostic test is available for an outbreak strain.


Original languageEnglish
Pages (from-to)1502-10
Number of pages9
JournalJAMA The Journal of the American Medical Association
Issue number14
Publication statusPublished - 10 Apr 2013


  • Computational Biology, DNA, Bacterial, Diarrhea, Disease Outbreaks, Escherichia coli Infections, Feces, Humans, Metagenomics, Retrospective Studies, Sensitivity and Specificity, Sequence Analysis, DNA, Shiga Toxin, Shiga-Toxigenic Escherichia coli, Time Factors