A critical role of T cell antigen receptor-transduced MHC class I-restricted helper T cells in tumor protection

Research output: Contribution to journalArticle

Authors

  • Emma C Morris
  • Aristotle Tsallios
  • Gavin Bendle
  • Shao-An Xue
  • Hans J Stauss

Colleges, School and Institutes

Abstract

Adoptive transfer of antigen-specific CD4(+) and CD8(+) T cells is one of the most efficient forms of cancer immunotherapy. However, the isolation of antigen-specific CD4(+) T cells is limited because only few tumor-associated helper epitopes are identified. Here, we used T cell antigen receptor gene transfer to target CD4(+) T cells against an MHC class I-presented epitope of a model tumor antigen. IFN-gamma-producing CD4(+) T cells were unable to expand in vivo and to provide help for tumor rejection. In contrast, CD4(+) T cells producing high levels of IL-2 expanded in vivo, provided help for cytotoxic T lymphocyte-mediated tumor rejection, and developed T cell memory. The data demonstrate in vivo synergy between T cell antigen receptor-transduced CD4(+) and CD8(+) T cells specific for the same epitope resulting in long-term tumor protection.

Details

Original languageEnglish
Pages (from-to)7934-9
Number of pages6
JournalNational Academy of Sciences. Proceedings
Volume102
Issue number22
Publication statusPublished - 31 May 2005

Keywords

  • Animals, Antigens, Neoplasm, CD8-Positive T-Lymphocytes, Epitopes, Histocompatibility Antigens Class I, Immunologic Memory, Immunotherapy, Adoptive, Interleukin-2, Mice, Mice, Inbred C57BL, Neoplasms, Receptors, Antigen, T-Cell, Retroviridae, Spleen, T-Lymphocytes, Helper-Inducer, Transduction, Genetic