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Abstract
We have assembled de novo the Escherichia coli K-12 MG1655 chromosome in a single 4.6-Mb contig using only nanopore data. Our method has three stages: (i) overlaps are detected between reads and then corrected by a multiple-alignment process; (ii) corrected reads are assembled using the Celera Assembler; and (iii) the assembly is polished using a probabilistic model of the signal-level data. The assembly reconstructs gene order and has 99.5% nucleotide identity.
Original language | English |
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Pages (from-to) | 733-5 |
Number of pages | 3 |
Journal | Nature Methods |
Volume | 12 |
Issue number | 8 |
DOIs | |
Publication status | Published - Aug 2015 |
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Dive into the research topics of 'A complete bacterial genome assembled de novo using only nanopore sequencing data'. Together they form a unique fingerprint.Projects
- 1 Finished
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Development of robust analytical pipelines for the analysis of microbial community data from clinical samples
1/09/12 → 31/08/15
Project: Research Councils