A complete bacterial genome assembled de novo using only nanopore sequencing data

Research output: Contribution to journalArticle

Authors

Colleges, School and Institutes

Abstract

We have assembled de novo the Escherichia coli K-12 MG1655 chromosome in a single 4.6-Mb contig using only nanopore data. Our method has three stages: (i) overlaps are detected between reads and then corrected by a multiple-alignment process; (ii) corrected reads are assembled using the Celera Assembler; and (iii) the assembly is polished using a probabilistic model of the signal-level data. The assembly reconstructs gene order and has 99.5% nucleotide identity.

Details

Original languageEnglish
Pages (from-to)733-5
Number of pages3
JournalNature Methods
Volume12
Issue number8
Publication statusPublished - Aug 2015