A compact, multidimensional spectrofluorometer exploiting supercontinuum generation
Research output: Contribution to journal › Article
Colleges, School and Institutes
We report a novel, compact and automated multidimensioual spectrofluorometer that exploits a fibre-laser-pumped ultrafast supercontinuum source to provide resolution with respect to intensity, excitation and emission wavelength, decay time and polarisation. This instrument has been applied to study the photophysics of the phase-sensitive membrane probe di-4-ANEPPDHQ and to characterise protein-protein interactions via Forster resonance energy transfer. It can be applied to in situ measurements via a fibre-optic probe in medical and other contexts and is demonstrated here to provide a comprehensive characterisation of tissue autofluorescence.
|Number of pages||12|
|Journal||Journal of Biophotonics|
|Early online date||1 Dec 2008|
|Publication status||E-pub ahead of print - 1 Dec 2008|