A compact, multidimensional spectrofluorometer exploiting supercontinuum generation

Research output: Contribution to journalArticle

Authors

  • Hugh B. Manning
  • Gordon T. Kennedy
  • David M. Grant
  • Anthony I. Magee
  • Mark A. A. Neil
  • Yoshifumi Itoh
  • Chris Dunsby
  • Paul M. W. French

Colleges, School and Institutes

Abstract

We report a novel, compact and automated multidimensioual spectrofluorometer that exploits a fibre-laser-pumped ultrafast supercontinuum source to provide resolution with respect to intensity, excitation and emission wavelength, decay time and polarisation. This instrument has been applied to study the photophysics of the phase-sensitive membrane probe di-4-ANEPPDHQ and to characterise protein-protein interactions via Forster resonance energy transfer. It can be applied to in situ measurements via a fibre-optic probe in medical and other contexts and is demonstrated here to provide a comprehensive characterisation of tissue autofluorescence.

Details

Original languageEnglish
Pages (from-to)494-505
Number of pages12
JournalJournal of Biophotonics
Volume1
Issue number6
Early online date1 Dec 2008
Publication statusE-pub ahead of print - 1 Dec 2008