A combination of NMR and liquid chromatography to characterize the protective effects of Rhus tripartita extracts on ethanol-induced toxicity and inflammation on intestinal cells

Research output: Contribution to journalArticle

Authors

  • Zaineb Ben Barka
  • Konstantinos Grintzalis
  • Madeleine Polet
  • Hanène Ben Miled
  • Khémais Ben Rhouma
  • Sakly Mohsen
  • Olfa Tebourbi
  • Yves-jacques Schneider

Colleges, School and Institutes

Abstract

Consumption of ethanol may have severe effects on human organs and tissues and lead to acute and chronic inflammation of internal organs. The present study aims at investigating the potential protective effects of three different extracts prepared from the leaves, root, and stem of the sumac, Rhus tripartita, against ethanol-induced toxicity and inflammation using intestinal cells as a cell culture system, in vitro model of the intestinal mucosa. The results showed an induction of cytotoxicity by ethanol, which was partially reversed by co-administration of the plant extracts. As part of investigating the cellular response and the mechanism of toxicity, the role of reduced thiols and glutathione-S-transferases were assessed. In addition, intestinal cells were artificially imposed to an inflammation state and the anti-inflammatory effect of the extracts was estimated by determination of interleukin-8. Finally, a detailed characterization of the contents of the three plant extracts by high resolution Nuclear Magnetic Resonance (NMR) spectroscopy and mass spectrometry revealed significant differences in their chemical compositions.

Details

Original languageEnglish
Pages (from-to)347-354
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume150
Early online date19 Dec 2017
Publication statusPublished - 20 Feb 2018

Keywords

  • Rhus tripartita extracts , NMR , LC-MS , ethanol toxicity , antioxidant , anti-inflammatory