TY - JOUR
T1 - A cell cycle-coordinated Polymerase II transcription compartment encompasses gene expression before global genome activation
AU - Hadzhiev, Yavor
AU - Qureshi, Haseeb K
AU - Wheatley, Lucy
AU - Cooper, Ledean
AU - Jasiulewicz, Aleksandra
AU - Van Nguyen, Huy
AU - Wragg, Joseph W
AU - Poovathumkadavil, Divyasree
AU - Conic, Sascha
AU - Bajan, Sarah
AU - Sik, Attila
AU - Hutvàgner, György
AU - Tora, Làszlò
AU - Gambus, Agnieszka
AU - Fossey, John S
AU - Müller, Ferenc
PY - 2019/2/11
Y1 - 2019/2/11
N2 - Most metazoan embryos commence development with rapid, transcriptionally silent cell divisions, with genome activation delayed until the mid-blastula transition (MBT). However, a set of genes escapes global repression and gets activated before MBT. Here we describe the formation and the spatio-temporal dynamics of a pair of distinct transcription compartments, which encompasses the earliest gene expression in zebrafish. 4D imaging of pri-miR430 and zinc-finger-gene activities by a novel, native transcription imaging approach reveals transcriptional sharing of nuclear compartments, which are regulated by homologous chromosome organisation. These compartments carry the majority of nascent-RNAs and active Polymerase II, are chromatin-depleted and represent the main sites of detectable transcription before MBT. Transcription occurs during the S-phase of increasingly permissive cleavage cycles. It is proposed, that the transcription compartment is part of the regulatory architecture of embryonic nuclei and offers a transcriptionally competent environment to facilitate early escape from repression before global genome activation.
AB - Most metazoan embryos commence development with rapid, transcriptionally silent cell divisions, with genome activation delayed until the mid-blastula transition (MBT). However, a set of genes escapes global repression and gets activated before MBT. Here we describe the formation and the spatio-temporal dynamics of a pair of distinct transcription compartments, which encompasses the earliest gene expression in zebrafish. 4D imaging of pri-miR430 and zinc-finger-gene activities by a novel, native transcription imaging approach reveals transcriptional sharing of nuclear compartments, which are regulated by homologous chromosome organisation. These compartments carry the majority of nascent-RNAs and active Polymerase II, are chromatin-depleted and represent the main sites of detectable transcription before MBT. Transcription occurs during the S-phase of increasingly permissive cleavage cycles. It is proposed, that the transcription compartment is part of the regulatory architecture of embryonic nuclei and offers a transcriptionally competent environment to facilitate early escape from repression before global genome activation.
UR - http://www.scopus.com/inward/record.url?scp=85061252272&partnerID=8YFLogxK
U2 - 10.1038/s41467-019-08487-5
DO - 10.1038/s41467-019-08487-5
M3 - Article
C2 - 30741925
SN - 2041-1723
VL - 10
SP - 691
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 691
ER -