A bifunctional triblock polynorbornene/carbon nanotube buckypaper bioelectrode for low-potential/high-current thionine-mediated glucose oxidation by FAD-GDH

We describe the fabrication of free-standing buckypaper bioelectrodes (BP) by co-immobilization of a FAD-dependent dehydrogenase and a thionine redox partner using either a pyreneNHS linker or a bifunctional triblock polynorbornene copolymer with pendant pyrene and NHS groups. While exhibiting excellent flexibility and conductivity, the BP bioelectrodes achieve high bioelectrocatalytic oxidation of glucose at onset potentials of -0.25 V vs. Ag/AgCl and maximum current densities of 3.7 mA cm^-2 at 50 mmol L^-1 glucose.