3D analysis of enamel demineralisation in human dental caries using high- resolution, large field of view synchrotron X-ray micro-computed tomography

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3D analysis of enamel demineralisation in human dental caries using high- resolution, large field of view synchrotron X-ray micro-computed tomography. / Besnard, Cyril; Harper, Robert; Moxham, Thomas; James, Jonathan; Storm, Malte; Salvati, Enrico; Landini, Gabriel; Shelton, Richard; Korsunsky, Alexander.

In: Materials Today Communications, Vol. 27, 102418, 06.2021.

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@article{5258510a470148e8979911e8bf6da93f,
title = "3D analysis of enamel demineralisation in human dental caries using high- resolution, large field of view synchrotron X-ray micro-computed tomography",
abstract = "We report major advances in the analysis of synchrotron 3D datasets acquired from human healthy and carious dental enamel. Synchrotron tomographic data for three human carious samples and a non-carious reference tooth sample were collected with the voxel size of 325 nm for a total volume of 815.4 × 815.4 × 685.4 μm3. The results were compared with conventional X-ray tomography, optical microscopy, and focused ion beam-scanning electron microscopy. Clear contrast was seen within demineralised enamel due to reduced mineral content using synchrotron tomography in comparison with conventional tomography. The features were found to correspond with the rod and inter-rod structures within prismatic enamel. 2D and 3D image segmentation allowed statistical quantification of important structural characteristics (such as the aspect ratio and the cross-sectional area of voids, as well as the demineralised volume fraction as a function of lesion depth). Whilst overall carious enamel predominantly displayed a Type 1 etching pattern (preferential demineralisation of enamel rods), a transition between Type 2 (preferential inter-rod demineralisation) and Type 1 was identified within the same lesion for the first time. This study does not provide extensive results on the different lesions studied, but illustrate a new method and its potential application.",
keywords = "Dental caries, Enamel, Demineralisation, Synchrotron, X-ray micro-computed tomography, Optical and (focused ion beam) scanning electron microscopy",
author = "Cyril Besnard and Robert Harper and Thomas Moxham and Jonathan James and Malte Storm and Enrico Salvati and Gabriel Landini and Richard Shelton and Alexander Korsunsky",
year = "2021",
month = jun,
doi = "10.1016/j.mtcomm.2021.102418",
language = "English",
volume = "27",
journal = "Materials Today Communications",
issn = "2352-4928",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - 3D analysis of enamel demineralisation in human dental caries using high- resolution, large field of view synchrotron X-ray micro-computed tomography

AU - Besnard, Cyril

AU - Harper, Robert

AU - Moxham, Thomas

AU - James, Jonathan

AU - Storm, Malte

AU - Salvati, Enrico

AU - Landini, Gabriel

AU - Shelton, Richard

AU - Korsunsky, Alexander

PY - 2021/6

Y1 - 2021/6

N2 - We report major advances in the analysis of synchrotron 3D datasets acquired from human healthy and carious dental enamel. Synchrotron tomographic data for three human carious samples and a non-carious reference tooth sample were collected with the voxel size of 325 nm for a total volume of 815.4 × 815.4 × 685.4 μm3. The results were compared with conventional X-ray tomography, optical microscopy, and focused ion beam-scanning electron microscopy. Clear contrast was seen within demineralised enamel due to reduced mineral content using synchrotron tomography in comparison with conventional tomography. The features were found to correspond with the rod and inter-rod structures within prismatic enamel. 2D and 3D image segmentation allowed statistical quantification of important structural characteristics (such as the aspect ratio and the cross-sectional area of voids, as well as the demineralised volume fraction as a function of lesion depth). Whilst overall carious enamel predominantly displayed a Type 1 etching pattern (preferential demineralisation of enamel rods), a transition between Type 2 (preferential inter-rod demineralisation) and Type 1 was identified within the same lesion for the first time. This study does not provide extensive results on the different lesions studied, but illustrate a new method and its potential application.

AB - We report major advances in the analysis of synchrotron 3D datasets acquired from human healthy and carious dental enamel. Synchrotron tomographic data for three human carious samples and a non-carious reference tooth sample were collected with the voxel size of 325 nm for a total volume of 815.4 × 815.4 × 685.4 μm3. The results were compared with conventional X-ray tomography, optical microscopy, and focused ion beam-scanning electron microscopy. Clear contrast was seen within demineralised enamel due to reduced mineral content using synchrotron tomography in comparison with conventional tomography. The features were found to correspond with the rod and inter-rod structures within prismatic enamel. 2D and 3D image segmentation allowed statistical quantification of important structural characteristics (such as the aspect ratio and the cross-sectional area of voids, as well as the demineralised volume fraction as a function of lesion depth). Whilst overall carious enamel predominantly displayed a Type 1 etching pattern (preferential demineralisation of enamel rods), a transition between Type 2 (preferential inter-rod demineralisation) and Type 1 was identified within the same lesion for the first time. This study does not provide extensive results on the different lesions studied, but illustrate a new method and its potential application.

KW - Dental caries

KW - Enamel

KW - Demineralisation

KW - Synchrotron

KW - X-ray micro-computed tomography

KW - Optical and (focused ion beam) scanning electron microscopy

U2 - 10.1016/j.mtcomm.2021.102418

DO - 10.1016/j.mtcomm.2021.102418

M3 - Article

VL - 27

JO - Materials Today Communications

JF - Materials Today Communications

SN - 2352-4928

M1 - 102418

ER -