Tim Knowles

ORCID: 0000-0002-6632-0985

Colleges, School and Institutes

Willingness to take PhD students

Yes

PhD projects

Dr Knowles is interested in supervising doctoral research students in the following areas:

The structural understanding of outer membrane protein biogenesis
The structural understanding of retrograde lipid transport in Gram-negative bacteria
Novel methods for the solubilisation of membrane proteins
The role of Cln3 in Batten Disease
γδ T cell selection

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Most downloaded publications

Cross-species chimeras reveal BamA POTRA and β-barrel domains must be fine-tuned for efficient OMP insertion
Doug Browning, Vass Bavro, Mason, J. L., Yanina Sevastsyanovich, Amanda Rossiter, Mark Jeeves, Timothy Wells, Tim Knowles, Adam Cunningham, Donald, J. W., Palmer, T., Michael Overduin & Ian Henderson, 12 Aug 2015, In : Molecular Microbiology. 97, 4, p. 646–659

Research output: Contribution to journal › Article

309 downloads
Membrane protein extraction and purification using styrene–maleic acid (SMA) copolymer: effect of variations in polymer structure
Morrison, K., Akram, A., Mathews, A., Khan, Z., Patel, J., Zhou, C., Hardy, D., Moore-Kelly, C., Patel, R., Obida, V., Tim Knowles, Javed, M., Chmel, N., Tim Dafforn & Rothnie, A., 25 Nov 2016, In : Biochemical Journal. 473, 23, p. 4349-4360

Research output: Contribution to journal › Article

199 downloads
Mutational and topological analysis of the Escherichia coli BamA protein
Doug Browning, Matthews, S. A., Amanda Rossiter, Yanina Sevastsyanovich, Mark Jeeves, Mason, J. L., Timothy Wells, Cathy Wardius, Tim Knowles, Adam Cunningham, Vass Bavro, Michael Overduin & Ian Henderson, 23 Dec 2013, In : PLoS ONE. 8, 12, e84512.

Research output: Contribution to journal › Article

175 downloads
MCE domain proteins: conserved inner membrane lipid-binding proteins required for outer membrane homeostasis
Georgia Isom, Nathaniel Davies, Chong, Z-S., Jack Bryant, Mohammed Jamshad, Sharif, M., Adam Cunningham, Tim Knowles, Chng, S-S., Jeff Cole & Ian Henderson, 17 Aug 2017, In : Scientific Reports. 7, 1, 8608.

Research output: Contribution to journal › Article

90 downloads
Encapsulated membrane proteins: a simplified system for molecular simulation
Sarah Lee, Khalid, S., Naomi Pollock, Tim Knowles, Edler, K., Rothnie, A. J., Owen Thomas & Tim Dafforn, Oct 2016, In : Biochimica et Biophysica Acta. 1858, 10, p. 2549-2557

Research output: Contribution to journal › Article

87 downloads

Recent publications

Evidence for phospholipid export from the bacterial inner membrane by the Mla ABC transport system
Hughes, G., Hall, S., Laxton, C., Sridhar, P., Mahadi, A., Hatton, C., Piggot, T., Wotherspoon, P., Leney, A., Ward, D., Jamshad, M., Spana, V., Cadby, I., Harding, C., Isom, G., Bryant, J., Parr, R., Yakub, Y., Jeeves, M., Huber, D. & 4 others, Ian Henderson, Clifton, L., Andrew Lovering & Tim Knowles, Oct 2019, In : Nature Microbiology. 4, p. 1692–1705

Research output: Contribution to journal › Article

SMA-PAGE: a new method to examine complexes of membrane proteins using SMALP nano-encapsulation and native gel electrophoresis
Pollock, N. L., Rai, M., Simon, K. S., Hesketh, S. J., Teo, A. C. K., Parmar, M., Sridhar, P., Collins, R., Lee, S. C., Stroud, Z. N., Bakker, S. E., Muench, S. P., Barton, C. H., Hurlbut, G., Roper, D. I., Smith, C. J. I., Knowles, T. J., Spickett, C. M., East, J. M., Postis, V. L. G. & 1 others, Tim Dafforn, 1 Aug 2019, In : Biochimica et Biophysica Acta (BBA) - Biomembranes. 1861, 8, p. 1437-1445 9 p.

Research output: Contribution to journal › Article

The C-terminal tail of the bacterial translocation ATPase SecA modulates its activity
Mohammed Jamshad, Tim Knowles, Scott White, Douglas Ward, Fiyaz Mohammed, Rahman, K. F., Wynne, M., Hughes, G. W., Kramer, G., Bukau, B. & Damon Huber, 27 Jun 2019, In : Elife. 8, 23 p., e48385.

Research output: Contribution to journal › Article

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Recent Activities

Rethinking the Mla pathway: Evidence for anterograde lipid trafficking in gram-negative bacteria
Tim Knowles (Chair)
24 Jun 2018

Activity: Talk or presentation › Invited talk

Rethinking the Mla pathway: Evidence for anterograde lipid trafficking in gram-negative bacteria
Tim Knowles (Chair)
12 Jun 2018

Activity: Talk or presentation › Invited talk

Viva Examination
Tim Knowles (Examiner), (Participant), (Examiner)
18 May 2018

Activity: Examination

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