Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA binding factor Rbm15/Spenito to the m6A machinery component Wtap/Fl(2)d

P Knuckles; T Lence; Irmgard Haussmann; D Jacob; N Kreim; SH Carl; I Masiello; T Hares; R Villasenior; D Hess; MA Andrade-Navarro; M Biggiogera; M Helm; Matthias Soller; M Buhler; JY Roignant

doi:10.1101/gad.309146.117

Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA binding factor Rbm15/Spenito to the m6A machinery component Wtap/Fl(2)d

P Knuckles, T Lence, Irmgard Haussmann, D Jacob, N Kreim, SH Carl, I Masiello, T Hares, R Villasenior, D Hess, MA Andrade-Navarro, M Biggiogera, M Helm, Matthias Soller, M Buhler, JY Roignant

Biosciences

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Abstract

N6-methyladenosine (m6A) is the most abundant mRNA modification in eukaryotes, playing crucial roles in multiple biological processes. m6A is catalyzed by the activity of Mettl3, which depends on additional proteins whose precise functions remain poorly understood. Here we identified Flacc/Zc3h13 as a novel interactor of m6A methyltransferase complex components in Drosophila and mouse. Like other components of this complex, Flacc controls m6A levels and is involved in sex determination in Drosophila. We demonstrate that Flacc promotes m6A deposition by bridging Fl(2)d to the mRNA binding factor Nito. Altogether, our work advances our molecular understanding of conservation and regulation of the m6A machinery.

Original language	English
Journal	Genes & Development
Early online date	13 Mar 2018
DOIs	https://doi.org/10.1101/gad.309146.117
Publication status	E-pub ahead of print - 13 Mar 2018

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Knuckles_et_al_Zc3h13_Flacc_Genes_&_DevelopmentFinal published version, 8.55 MBLicence: Creative Commons: Attribution (CC BY)

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Knuckles, P., Lence, T., Haussmann, I., Jacob, D., Kreim, N., Carl, SH., Masiello, I., Hares, T., Villasenior, R., Hess, D., Andrade-Navarro, MA., Biggiogera, M., Helm, M., Soller, M., Buhler, M., & Roignant, JY. (2018). Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA binding factor Rbm15/Spenito to the m6A machinery component Wtap/Fl(2)d. Genes & Development. Advance online publication. https://doi.org/10.1101/gad.309146.117

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title = "Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA binding factor Rbm15/Spenito to the m6A machinery component Wtap/Fl(2)d",

abstract = "N6-methyladenosine (m6A) is the most abundant mRNA modification in eukaryotes, playing crucial roles in multiple biological processes. m6A is catalyzed by the activity of Mettl3, which depends on additional proteins whose precise functions remain poorly understood. Here we identified Flacc/Zc3h13 as a novel interactor of m6A methyltransferase complex components in Drosophila and mouse. Like other components of this complex, Flacc controls m6A levels and is involved in sex determination in Drosophila. We demonstrate that Flacc promotes m6A deposition by bridging Fl(2)d to the mRNA binding factor Nito. Altogether, our work advances our molecular understanding of conservation and regulation of the m6A machinery.",

author = "P Knuckles and T Lence and Irmgard Haussmann and D Jacob and N Kreim and SH Carl and I Masiello and T Hares and R Villasenior and D Hess and MA Andrade-Navarro and M Biggiogera and M Helm and Matthias Soller and M Buhler and JY Roignant",

year = "2018",

month = mar,

day = "13",

doi = "10.1101/gad.309146.117",

language = "English",

journal = "Genes & Development",

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publisher = "Cold Spring Harbor Laboratory Press",

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Knuckles, P, Lence, T, Haussmann, I, Jacob, D, Kreim, N, Carl, SH, Masiello, I, Hares, T, Villasenior, R, Hess, D, Andrade-Navarro, MA, Biggiogera, M, Helm, M, Soller, M, Buhler, M & Roignant, JY 2018, 'Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA binding factor Rbm15/Spenito to the m6A machinery component Wtap/Fl(2)d', Genes & Development. https://doi.org/10.1101/gad.309146.117

TY - JOUR

T1 - Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA binding factor Rbm15/Spenito to the m6A machinery component Wtap/Fl(2)d

AU - Knuckles, P

AU - Lence, T

AU - Haussmann, Irmgard

AU - Jacob, D

AU - Kreim, N

AU - Carl, SH

AU - Masiello, I

AU - Hares, T

AU - Villasenior, R

AU - Hess, D

AU - Andrade-Navarro, MA

AU - Biggiogera, M

AU - Helm, M

AU - Soller, Matthias

AU - Buhler, M

AU - Roignant, JY

PY - 2018/3/13

Y1 - 2018/3/13

N2 - N6-methyladenosine (m6A) is the most abundant mRNA modification in eukaryotes, playing crucial roles in multiple biological processes. m6A is catalyzed by the activity of Mettl3, which depends on additional proteins whose precise functions remain poorly understood. Here we identified Flacc/Zc3h13 as a novel interactor of m6A methyltransferase complex components in Drosophila and mouse. Like other components of this complex, Flacc controls m6A levels and is involved in sex determination in Drosophila. We demonstrate that Flacc promotes m6A deposition by bridging Fl(2)d to the mRNA binding factor Nito. Altogether, our work advances our molecular understanding of conservation and regulation of the m6A machinery.

AB - N6-methyladenosine (m6A) is the most abundant mRNA modification in eukaryotes, playing crucial roles in multiple biological processes. m6A is catalyzed by the activity of Mettl3, which depends on additional proteins whose precise functions remain poorly understood. Here we identified Flacc/Zc3h13 as a novel interactor of m6A methyltransferase complex components in Drosophila and mouse. Like other components of this complex, Flacc controls m6A levels and is involved in sex determination in Drosophila. We demonstrate that Flacc promotes m6A deposition by bridging Fl(2)d to the mRNA binding factor Nito. Altogether, our work advances our molecular understanding of conservation and regulation of the m6A machinery.

U2 - 10.1101/gad.309146.117

DO - 10.1101/gad.309146.117

M3 - Article

SN - 0890-9369

JO - Genes & Development

JF - Genes & Development

ER -

Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA binding factor Rbm15/Spenito to the m6A machinery component Wtap/Fl(2)d

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