X-ray holographic microscopy with zone plates applied to biological samples in the water window using 3rd harmonic radiation from the free-electron laser FLASH

T Gorniak; R Heine; AP Mancuso; F Staier; C Christophis; Michala Pettitt; A Sakdinawat; R Treusch; N Guerassimova; J Feldhaus; C Gutt; G Gruebel; S Eisebitt; A Beyer; A Goelzhaeuser; E Weckert; M Grunze; IA Vartanyants; A Rosenhahn

X-ray holographic microscopy with zone plates applied to biological samples in the water window using 3rd harmonic radiation from the free-electron laser FLASH

T Gorniak, R Heine, AP Mancuso, F Staier, C Christophis, Michala Pettitt, A Sakdinawat, R Treusch, N Guerassimova, J Feldhaus, C Gutt, G Gruebel, S Eisebitt, A Beyer, A Goelzhaeuser, E Weckert, M Grunze, IA Vartanyants, A Rosenhahn

Research output: Contribution to journal › Article

33 Citations (Scopus)

Abstract

The imaging of hydrated biological samples - especially in the energy window of 284-540 eV, where water does not obscure the signal of soft organic matter and biologically relevant elements - is of tremendous interest for life sciences. Free-electron lasers can provide highly intense and coherent pulses, which allow single pulse imaging to overcome resolution limits set by radiation damage. One current challenge is to match both the desired energy and the intensity of the light source. We present the first images of dehydrated biological material acquired with 3rd harmonic radiation from FLASH by digital in-line zone plate holography as one step towards the vision of imaging hydrated biological material with photons in the water window. We also demonstrate the first application of ultrathin molecular sheets as suitable substrates for future free-electron laser experiments with biological samples in the form of a rat fibroblast cell and marine biofouling bacteria Cobetia marina. (C) 2011 Optical Society of America

Original language	English
Pages (from-to)	11059-11070
Number of pages	12
Journal	Optics Express
Volume	19
Issue number	12
Publication status	Published - 1 Jun 2011

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Gorniak, T., Heine, R., Mancuso, AP., Staier, F., Christophis, C., Pettitt, M., Sakdinawat, A., Treusch, R., Guerassimova, N., Feldhaus, J., Gutt, C., Gruebel, G., Eisebitt, S., Beyer, A., Goelzhaeuser, A., Weckert, E., Grunze, M., Vartanyants, IA., & Rosenhahn, A. (2011). X-ray holographic microscopy with zone plates applied to biological samples in the water window using 3rd harmonic radiation from the free-electron laser FLASH. Optics Express, 19(12), 11059-11070.

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title = "X-ray holographic microscopy with zone plates applied to biological samples in the water window using 3rd harmonic radiation from the free-electron laser FLASH",

abstract = "The imaging of hydrated biological samples - especially in the energy window of 284-540 eV, where water does not obscure the signal of soft organic matter and biologically relevant elements - is of tremendous interest for life sciences. Free-electron lasers can provide highly intense and coherent pulses, which allow single pulse imaging to overcome resolution limits set by radiation damage. One current challenge is to match both the desired energy and the intensity of the light source. We present the first images of dehydrated biological material acquired with 3rd harmonic radiation from FLASH by digital in-line zone plate holography as one step towards the vision of imaging hydrated biological material with photons in the water window. We also demonstrate the first application of ultrathin molecular sheets as suitable substrates for future free-electron laser experiments with biological samples in the form of a rat fibroblast cell and marine biofouling bacteria Cobetia marina. (C) 2011 Optical Society of America",

author = "T Gorniak and R Heine and AP Mancuso and F Staier and C Christophis and Michala Pettitt and A Sakdinawat and R Treusch and N Guerassimova and J Feldhaus and C Gutt and G Gruebel and S Eisebitt and A Beyer and A Goelzhaeuser and E Weckert and M Grunze and IA Vartanyants and A Rosenhahn",

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Gorniak, T, Heine, R, Mancuso, AP, Staier, F, Christophis, C, Pettitt, M, Sakdinawat, A, Treusch, R, Guerassimova, N, Feldhaus, J, Gutt, C, Gruebel, G, Eisebitt, S, Beyer, A, Goelzhaeuser, A, Weckert, E, Grunze, M, Vartanyants, IA & Rosenhahn, A 2011, 'X-ray holographic microscopy with zone plates applied to biological samples in the water window using 3rd harmonic radiation from the free-electron laser FLASH', Optics Express, vol. 19, no. 12, pp. 11059-11070.

TY - JOUR

T1 - X-ray holographic microscopy with zone plates applied to biological samples in the water window using 3rd harmonic radiation from the free-electron laser FLASH

AU - Gorniak, T

AU - Heine, R

AU - Mancuso, AP

AU - Staier, F

AU - Christophis, C

AU - Pettitt, Michala

AU - Sakdinawat, A

AU - Treusch, R

AU - Guerassimova, N

AU - Feldhaus, J

AU - Gutt, C

AU - Gruebel, G

AU - Eisebitt, S

AU - Beyer, A

AU - Goelzhaeuser, A

AU - Weckert, E

AU - Grunze, M

AU - Vartanyants, IA

AU - Rosenhahn, A

PY - 2011/6/1

Y1 - 2011/6/1

N2 - The imaging of hydrated biological samples - especially in the energy window of 284-540 eV, where water does not obscure the signal of soft organic matter and biologically relevant elements - is of tremendous interest for life sciences. Free-electron lasers can provide highly intense and coherent pulses, which allow single pulse imaging to overcome resolution limits set by radiation damage. One current challenge is to match both the desired energy and the intensity of the light source. We present the first images of dehydrated biological material acquired with 3rd harmonic radiation from FLASH by digital in-line zone plate holography as one step towards the vision of imaging hydrated biological material with photons in the water window. We also demonstrate the first application of ultrathin molecular sheets as suitable substrates for future free-electron laser experiments with biological samples in the form of a rat fibroblast cell and marine biofouling bacteria Cobetia marina. (C) 2011 Optical Society of America

AB - The imaging of hydrated biological samples - especially in the energy window of 284-540 eV, where water does not obscure the signal of soft organic matter and biologically relevant elements - is of tremendous interest for life sciences. Free-electron lasers can provide highly intense and coherent pulses, which allow single pulse imaging to overcome resolution limits set by radiation damage. One current challenge is to match both the desired energy and the intensity of the light source. We present the first images of dehydrated biological material acquired with 3rd harmonic radiation from FLASH by digital in-line zone plate holography as one step towards the vision of imaging hydrated biological material with photons in the water window. We also demonstrate the first application of ultrathin molecular sheets as suitable substrates for future free-electron laser experiments with biological samples in the form of a rat fibroblast cell and marine biofouling bacteria Cobetia marina. (C) 2011 Optical Society of America

M3 - Article

C2 - 21716334

VL - 19

SP - 11059

EP - 11070

JO - Optics Express

JF - Optics Express

IS - 12

ER -

X-ray holographic microscopy with zone plates applied to biological samples in the water window using 3rd harmonic radiation from the free-electron laser FLASH

Abstract

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