Visualisation of metal deposition in biofilm reactors by three-dimensional magnetic resonance imaging (MRI)

KP Nott, Marion Paterson-Beedle, Lynne Macaskie, LD Hall

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Three-dimensional magnetic resonance imaging (MRI) was used to visualise polyurethane foam-immobilised Citrobacter after challenging with La3+ and/or Cu2+ in citrate buffer supplemented with glycerol 2-phosphate. Extensive phosphatase-mediated bioaccumulation of LaPO4 was observed but no evidence for deposition of Cu-3(PO4)(2) was obtained by X-ray diffraction and proton-induced X-ray emission analyses. Image analysis showed that La3+/Cu2+ is a good model system to study the function of this biofilm reactor non-invasively by MRI.
Original languageEnglish
Pages (from-to)1749-1757
Number of pages9
JournalBiotechnology Letters
Volume23
Issue number21
Publication statusPublished - 1 Jan 2001

Keywords

  • lanthanum
  • biofilm
  • copper
  • Citrobacter
  • magnetic resonance imaging
  • bioreactor

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