Versatile, cell and chip friendly method to gel alginate in microfluidic devices

Armend G. Håti, David Bassett, Jonas M. Ribe, Pawel Sikorski, David A. Weitz, Bjørn T. Stokke

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

Alginate is used extensively in microfluidic devices to produce discrete beads or fibres at the microscale. Such structures may be used to encapsulate sensitive cargoes such as cells and biomolecules. On chip gelation of alginate represents a significant challenge since gelling kinetics or physicochemical conditions are not biocompatible. Here we present a new method that offers a hitherto unprecedented level of control over the gelling kinetics and pH applied to the encapsulation of a variety of cells in both bead and fibre geometries. This versatile approach proved straightforward to adjust to achieve appropriate solution conditions required for implementation in microfluidic devices and resulted in highly reliable device operation and very high viability of several different encapsulated cell types for prolonged periods. We believe this method offers a paradigm shift in alginate gelling technology for application in microfluidics.

Original languageEnglish
Pages (from-to)3718-3727
Number of pages10
JournalLab on a Chip
Volume16
Issue number19
DOIs
Publication statusPublished - 11 Aug 2016

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Biochemistry
  • Biomedical Engineering

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