TY - JOUR
T1 - Vascular Adhesion Protein-1 Determines the Cellular Properties of Endometrial Pericytes
AU - Gharanei, Seley
AU - Fishwick, Katherine
AU - Peter Durairaj, Ruban
AU - Jin, Tianrong
AU - Siamantouras, Eleftherios
AU - Liu, Kuo-Kang
AU - Straube, Anne
AU - Lucas, Emma S
AU - Weston, Christopher J
AU - Rantakari, Pia
AU - Salmi, Marko
AU - Jalkanen, Sirpa
AU - Brosens, Jan J
AU - Tan, Bee Kang
N1 - Copyright © 2021 Gharanei, Fishwick, Peter Durairaj, Jin, Siamantouras, Liu, Straube, Lucas, Weston, Rantakari, Salmi, Jalkanen, Brosens and Tan.
PY - 2020
Y1 - 2020
N2 - Vascular adhesion protein-1 (VAP-1) is an inflammation-inducible adhesion molecule and a primary amine oxidase involved in immune cell trafficking. Leukocyte extravasation into tissues is mediated by adhesion molecules expressed on endothelial cells and pericytes. Pericytes play a major role in the angiogenesis and vascularization of cycling endometrium. However, the functional properties of pericytes in the human endometrium are not known. Here we show that pericytes surrounding the spiral arterioles in midluteal human endometrium constitutively express VAP-1. We first characterize these pericytes and demonstrate that knockdown of VAP-1 perturbed their biophysical properties and compromised their contractile, migratory, adhesive and clonogenic capacities. Furthermore, we show that loss of VAP-1 disrupts pericyte-uterine natural killer cell interactions in vitro. Taken together, the data not only reveal that endometrial pericytes represent a cell population with distinct biophysical and functional properties but also suggest a pivotal role for VAP-1 in regulating the recruitment of innate immune cells in human endometrium. We posit that VAP-1 could serve as a potential biomarker for pregnancy pathologies caused by a compromised perivascular environment prior to conception.
AB - Vascular adhesion protein-1 (VAP-1) is an inflammation-inducible adhesion molecule and a primary amine oxidase involved in immune cell trafficking. Leukocyte extravasation into tissues is mediated by adhesion molecules expressed on endothelial cells and pericytes. Pericytes play a major role in the angiogenesis and vascularization of cycling endometrium. However, the functional properties of pericytes in the human endometrium are not known. Here we show that pericytes surrounding the spiral arterioles in midluteal human endometrium constitutively express VAP-1. We first characterize these pericytes and demonstrate that knockdown of VAP-1 perturbed their biophysical properties and compromised their contractile, migratory, adhesive and clonogenic capacities. Furthermore, we show that loss of VAP-1 disrupts pericyte-uterine natural killer cell interactions in vitro. Taken together, the data not only reveal that endometrial pericytes represent a cell population with distinct biophysical and functional properties but also suggest a pivotal role for VAP-1 in regulating the recruitment of innate immune cells in human endometrium. We posit that VAP-1 could serve as a potential biomarker for pregnancy pathologies caused by a compromised perivascular environment prior to conception.
U2 - 10.3389/fcell.2020.621016
DO - 10.3389/fcell.2020.621016
M3 - Article
C2 - 33537312
SN - 2296-634X
VL - 8
SP - 621016
JO - Frontiers in cell and developmental biology
JF - Frontiers in cell and developmental biology
ER -