Using tg(Vtg1:Mcherry) zebrafish embryos to test the estrogenic effects of endocrine disrupting compounds

Zsolt Csenki, Ákos Horváth, Illés Bock, Edina Garai, Flóra Kerekes, Erna Vásárhelyi, Balázs Kovács, Béla Urbányi, Ferenc Mueller, Katalin Bakos

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

There are many endocrine disrupting compounds (EDC) in the environment, especially estrogenic substances. The detection of these substances is difficult due to their chemical diversity; therefore, increasingly more effect-detecting methods are used, such as estrogenic effect-sensitive biomonitor/bioindicator organisms. These biomonitoring organisms include several fish models. This protocol covers the use of zebrafish Tg(vtg1: mCherry) transgenic line as a biomonitoring organism, including the propagation of fish and the treatment of embryos, with an emphasis on the detection, documentation, and evaluation of fluorescent signals induced by EDC. The goal of the work is the demonstration of the use of the Tg(vtg1: mCherry) transgenic line embryos to detect estrogenic effects. This work documents the use of transgenic zebrafish embryos Tg(vtg1: mCherry) for the detection of estrogenic effects by testing two estrogenic substances, α-and β-zearalenol. The described protocol is only a basis for designing assays; the test method can be varied according to the test endpoints and the samples. Moreover, it can be combined with other assay methods, thereby facilitating the future use of the transgenic line.

Original languageEnglish
Article numbere60462
Pages (from-to)1-15
Number of pages15
JournalJournal of Visualized Experiments
Volume2020
Issue number162
DOIs
Publication statusPublished - 2020

Bibliographical note

Funding Information:
This work was supported by the National Research, Development and Innovation Office (NKFIH) from the National Research, Development and Innovation Fund (NKFIA); Grant Agreement: NVKP_16-1-2016-0003, EFOP-3.6.3-VEKOP-16-2017-00008 project co-financed by the European Union, and the Thematic Excellence Program NKFIH-831-10/2019 of Szent Istv?n University, awarded by Ministry for Innovation and Technology.

Funding Information:
This work was supported by the National Research, Development and Innovation Office (NKFIH) from the National Research, Development and Innovation Fund (NKFIA); Grant Agreement: NVKP_16-1-2016-0003, EFOP-3.6.3-VEKOP-16-2017-00008 project co-financed by the European Union, and the Thematic Excellence Program NKFIH-831-10/2019 of Szent István University, awarded by Ministry for Innovation and Technology.

Publisher Copyright:
© 2020 JoVE Journal of Visualized Experiments.

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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