Using tg(Vtg1:Mcherry) zebrafish embryos to test the estrogenic effects of endocrine disrupting compounds

Zsolt Csenki; Ákos Horváth; Illés Bock; Edina Garai; Flóra Kerekes; Erna Vásárhelyi; Balázs Kovács; Béla Urbányi; Ferenc Mueller; Katalin Bakos

doi:10.3791/60462

Using tg(Vtg1:Mcherry) zebrafish embryos to test the estrogenic effects of endocrine disrupting compounds

Zsolt Csenki^*, Ákos Horváth, Illés Bock, Edina Garai, Flóra Kerekes, Erna Vásárhelyi, Balázs Kovács, Béla Urbányi, Ferenc Mueller, Katalin Bakos

^*Corresponding author for this work

Cancer and Genomic Sciences

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

There are many endocrine disrupting compounds (EDC) in the environment, especially estrogenic substances. The detection of these substances is difficult due to their chemical diversity; therefore, increasingly more effect-detecting methods are used, such as estrogenic effect-sensitive biomonitor/bioindicator organisms. These biomonitoring organisms include several fish models. This protocol covers the use of zebrafish Tg(vtg1: mCherry) transgenic line as a biomonitoring organism, including the propagation of fish and the treatment of embryos, with an emphasis on the detection, documentation, and evaluation of fluorescent signals induced by EDC. The goal of the work is the demonstration of the use of the Tg(vtg1: mCherry) transgenic line embryos to detect estrogenic effects. This work documents the use of transgenic zebrafish embryos Tg(vtg1: mCherry) for the detection of estrogenic effects by testing two estrogenic substances, α-and β-zearalenol. The described protocol is only a basis for designing assays; the test method can be varied according to the test endpoints and the samples. Moreover, it can be combined with other assay methods, thereby facilitating the future use of the transgenic line.

Original language	English
Article number	e60462
Pages (from-to)	1-15
Number of pages	15
Journal	Journal of Visualized Experiments
Volume	2020
Issue number	162
DOIs	https://doi.org/10.3791/60462
Publication status	Published - 2020

Bibliographical note

Funding Information:
This work was supported by the National Research, Development and Innovation Office (NKFIH) from the National Research, Development and Innovation Fund (NKFIA); Grant Agreement: NVKP_16-1-2016-0003, EFOP-3.6.3-VEKOP-16-2017-00008 project co-financed by the European Union, and the Thematic Excellence Program NKFIH-831-10/2019 of Szent Istv?n University, awarded by Ministry for Innovation and Technology.

Funding Information:
This work was supported by the National Research, Development and Innovation Office (NKFIH) from the National Research, Development and Innovation Fund (NKFIA); Grant Agreement: NVKP_16-1-2016-0003, EFOP-3.6.3-VEKOP-16-2017-00008 project co-financed by the European Union, and the Thematic Excellence Program NKFIH-831-10/2019 of Szent István University, awarded by Ministry for Innovation and Technology.

Publisher Copyright:
© 2020 JoVE Journal of Visualized Experiments.

ASJC Scopus subject areas

General Neuroscience
General Chemical Engineering
General Biochemistry,Genetics and Molecular Biology
General Immunology and Microbiology

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Cite this

@article{26caf0bc8f8942899a4f7ba178bd1405,

title = "Using tg(Vtg1:Mcherry) zebrafish embryos to test the estrogenic effects of endocrine disrupting compounds",

abstract = "There are many endocrine disrupting compounds (EDC) in the environment, especially estrogenic substances. The detection of these substances is difficult due to their chemical diversity; therefore, increasingly more effect-detecting methods are used, such as estrogenic effect-sensitive biomonitor/bioindicator organisms. These biomonitoring organisms include several fish models. This protocol covers the use of zebrafish Tg(vtg1: mCherry) transgenic line as a biomonitoring organism, including the propagation of fish and the treatment of embryos, with an emphasis on the detection, documentation, and evaluation of fluorescent signals induced by EDC. The goal of the work is the demonstration of the use of the Tg(vtg1: mCherry) transgenic line embryos to detect estrogenic effects. This work documents the use of transgenic zebrafish embryos Tg(vtg1: mCherry) for the detection of estrogenic effects by testing two estrogenic substances, α-and β-zearalenol. The described protocol is only a basis for designing assays; the test method can be varied according to the test endpoints and the samples. Moreover, it can be combined with other assay methods, thereby facilitating the future use of the transgenic line.",

author = "Zsolt Csenki and {\'A}kos Horv{\'a}th and Ill{\'e}s Bock and Edina Garai and Fl{\'o}ra Kerekes and Erna V{\'a}s{\'a}rhelyi and Bal{\'a}zs Kov{\'a}cs and B{\'e}la Urb{\'a}nyi and Ferenc Mueller and Katalin Bakos",

note = "Funding Information: This work was supported by the National Research, Development and Innovation Office (NKFIH) from the National Research, Development and Innovation Fund (NKFIA); Grant Agreement: NVKP_16-1-2016-0003, EFOP-3.6.3-VEKOP-16-2017-00008 project co-financed by the European Union, and the Thematic Excellence Program NKFIH-831-10/2019 of Szent Istv?n University, awarded by Ministry for Innovation and Technology. Funding Information: This work was supported by the National Research, Development and Innovation Office (NKFIH) from the National Research, Development and Innovation Fund (NKFIA); Grant Agreement: NVKP_16-1-2016-0003, EFOP-3.6.3-VEKOP-16-2017-00008 project co-financed by the European Union, and the Thematic Excellence Program NKFIH-831-10/2019 of Szent Istv{\'a}n University, awarded by Ministry for Innovation and Technology. Publisher Copyright: {\textcopyright} 2020 JoVE Journal of Visualized Experiments.",

year = "2020",

doi = "10.3791/60462",

language = "English",

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journal = "Journal of Visualized Experiments",

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AU - Csenki, Zsolt

AU - Horváth, Ákos

AU - Bock, Illés

AU - Garai, Edina

AU - Kerekes, Flóra

AU - Vásárhelyi, Erna

AU - Kovács, Balázs

AU - Urbányi, Béla

AU - Mueller, Ferenc

AU - Bakos, Katalin

N1 - Funding Information: This work was supported by the National Research, Development and Innovation Office (NKFIH) from the National Research, Development and Innovation Fund (NKFIA); Grant Agreement: NVKP_16-1-2016-0003, EFOP-3.6.3-VEKOP-16-2017-00008 project co-financed by the European Union, and the Thematic Excellence Program NKFIH-831-10/2019 of Szent Istv?n University, awarded by Ministry for Innovation and Technology. Funding Information: This work was supported by the National Research, Development and Innovation Office (NKFIH) from the National Research, Development and Innovation Fund (NKFIA); Grant Agreement: NVKP_16-1-2016-0003, EFOP-3.6.3-VEKOP-16-2017-00008 project co-financed by the European Union, and the Thematic Excellence Program NKFIH-831-10/2019 of Szent István University, awarded by Ministry for Innovation and Technology. Publisher Copyright: © 2020 JoVE Journal of Visualized Experiments.

PY - 2020

Y1 - 2020

N2 - There are many endocrine disrupting compounds (EDC) in the environment, especially estrogenic substances. The detection of these substances is difficult due to their chemical diversity; therefore, increasingly more effect-detecting methods are used, such as estrogenic effect-sensitive biomonitor/bioindicator organisms. These biomonitoring organisms include several fish models. This protocol covers the use of zebrafish Tg(vtg1: mCherry) transgenic line as a biomonitoring organism, including the propagation of fish and the treatment of embryos, with an emphasis on the detection, documentation, and evaluation of fluorescent signals induced by EDC. The goal of the work is the demonstration of the use of the Tg(vtg1: mCherry) transgenic line embryos to detect estrogenic effects. This work documents the use of transgenic zebrafish embryos Tg(vtg1: mCherry) for the detection of estrogenic effects by testing two estrogenic substances, α-and β-zearalenol. The described protocol is only a basis for designing assays; the test method can be varied according to the test endpoints and the samples. Moreover, it can be combined with other assay methods, thereby facilitating the future use of the transgenic line.

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AN - SCOPUS:85090072146

SN - 1940-087X

VL - 2020

SP - 1

EP - 15

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

IS - 162

M1 - e60462

ER -

Using tg(Vtg1:Mcherry) zebrafish embryos to test the estrogenic effects of endocrine disrupting compounds

Abstract

Bibliographical note

ASJC Scopus subject areas

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