Using a non-invasive assessment of lung injury in a murine model of acute lung injury

S. Lax; M. Takata; D. R. Thickett; Michael R Wilson

doi:10.1136/bmjresp-2013-000014

Using a non-invasive assessment of lung injury in a murine model of acute lung injury

S. Lax, M. Takata, D. R. Thickett, Michael R Wilson

Cardiovascular Sciences

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Abstract

Arterial oxygen saturation has not been assessed sequentially in conscious mice as a direct consequence of an in vivo murine model of acute lung injury. Here, we report daily changes in arterial oxygen saturation and other cardiopulmonary parameters by using infrared pulse oximetry following intratracheal lipopolysaccharide (IT-LPS) for up to 9 days, and following IT-phosphate buffered saline up to 72 h as a control. We show that arterial oxygen saturation decreases, with maximal decline at 96 h post IT-LPS. Blood oxygen levels negatively correlate with 7 of 10 quantitative markers of murine lung injury, including neutrophilia and interleukin-6 expression. This identifies infrared pulse oximetry as a method to non-invasively monitor arterial oxygen saturation following direct LPS instillations.

Original language	English
Article number	e000014
Journal	BMJ Open Respiratory Research
Volume	1
Issue number	1
DOIs	https://doi.org/10.1136/bmjresp-2013-000014
Publication status	Published - 3 Jan 2014

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10.1136/bmjresp-2013-000014

Lax_Thickett_Using_non_invasive_assessment_BMJ_Open_Resp_Res_2014
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http://bmjopenrespres.bmj.com/lookup/doi/10.1136/bmjresp-2013-000014

The Functional Importance of Dysregulated Alveolar Steroid Metabolism in Acute and Resolving Lung Injury
Thickett, D. (Principal Investigator), Cooper, M. (Co-Investigator) & Stewart, P. (Co-Investigator)
Wellcome Trust
8/01/11 → 7/01/14
Project: Research

Cite this

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title = "Using a non-invasive assessment of lung injury in a murine model of acute lung injury",

abstract = "Arterial oxygen saturation has not been assessed sequentially in conscious mice as a direct consequence of an in vivo murine model of acute lung injury. Here, we report daily changes in arterial oxygen saturation and other cardiopulmonary parameters by using infrared pulse oximetry following intratracheal lipopolysaccharide (IT-LPS) for up to 9 days, and following IT-phosphate buffered saline up to 72 h as a control. We show that arterial oxygen saturation decreases, with maximal decline at 96 h post IT-LPS. Blood oxygen levels negatively correlate with 7 of 10 quantitative markers of murine lung injury, including neutrophilia and interleukin-6 expression. This identifies infrared pulse oximetry as a method to non-invasively monitor arterial oxygen saturation following direct LPS instillations.",

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AU - Takata, M.

AU - Thickett, D. R.

AU - Wilson, Michael R

PY - 2014/1/3

Y1 - 2014/1/3

N2 - Arterial oxygen saturation has not been assessed sequentially in conscious mice as a direct consequence of an in vivo murine model of acute lung injury. Here, we report daily changes in arterial oxygen saturation and other cardiopulmonary parameters by using infrared pulse oximetry following intratracheal lipopolysaccharide (IT-LPS) for up to 9 days, and following IT-phosphate buffered saline up to 72 h as a control. We show that arterial oxygen saturation decreases, with maximal decline at 96 h post IT-LPS. Blood oxygen levels negatively correlate with 7 of 10 quantitative markers of murine lung injury, including neutrophilia and interleukin-6 expression. This identifies infrared pulse oximetry as a method to non-invasively monitor arterial oxygen saturation following direct LPS instillations.

AB - Arterial oxygen saturation has not been assessed sequentially in conscious mice as a direct consequence of an in vivo murine model of acute lung injury. Here, we report daily changes in arterial oxygen saturation and other cardiopulmonary parameters by using infrared pulse oximetry following intratracheal lipopolysaccharide (IT-LPS) for up to 9 days, and following IT-phosphate buffered saline up to 72 h as a control. We show that arterial oxygen saturation decreases, with maximal decline at 96 h post IT-LPS. Blood oxygen levels negatively correlate with 7 of 10 quantitative markers of murine lung injury, including neutrophilia and interleukin-6 expression. This identifies infrared pulse oximetry as a method to non-invasively monitor arterial oxygen saturation following direct LPS instillations.

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DO - 10.1136/bmjresp-2013-000014

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JO - BMJ Open Respiratory Research

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Using a non-invasive assessment of lung injury in a murine model of acute lung injury

Abstract

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The Functional Importance of Dysregulated Alveolar Steroid Metabolism in Acute and Resolving Lung Injury

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