Projects per year
Abstract
High level expression of a recombinant gene results in growth arrest, followed by overgrowth by non-productive derivatives. Two methods are described for the isolation of E. coli BL21* strains that are improved hosts for recombinant protein production. Both are based upon the observations (i) that fluorescence of a C-terminal GFP tag is a reliable reporter of the production and correct folding of the N-terminal target domain; and (ii) rare mutants arise spontaneously that remain productive during long periods of high level recombinant protein production. The first method relies upon identifying these mutants amongst colonies on agar plates; the other exploits fluorescence activated cell sorting. Although identical mutations in the regulatory region of the T7 polymerase gene were found in all of the improved host strains isolated, they differed in their ability to accumulate the outer membrane protein, Ccp, or a cytoplasmic protein, CheY-GFP. Cytochrome c peroxidase activity of recombinant Ccp from one of these strains was demonstrated. Changes in levels of T7 polymerase expression are therefore insufficient to ensure increased accumulation of all recombinant proteins. We demonstrate that the methods described allow strains to be isolated that carry other, currently uncharacterised mutations that are required depending on the target protein.
Original language | English |
---|---|
Pages (from-to) | 11-21 |
Number of pages | 11 |
Journal | Journal of Biotechnology |
Volume | 156 |
Issue number | 1 |
DOIs | |
Publication status | Published - 20 Oct 2011 |
Fingerprint
Dive into the research topics of 'Use of GFP fusions for the isolation of Escherichia coli strains for improved production of different target recombinant proteins.'. Together they form a unique fingerprint.Projects
- 1 Finished
-
Microbial physiology underpinning the production of difficult recombiinant proteins
Cole, J., Overton, T. & Hewitt, C.
Biotechnology & Biological Sciences Research Council
1/10/06 → 30/09/09
Project: Research Councils