Use of explant technology in the study of in vitro immune responses

Graham Anderson*, Eric J. Jenkinson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The establishment of in vitro culture systems provides an accessible means to study events within the immune system. In contrast to either dispersed suspension or two-dimensional monolayer culture, the explantation of tissue fragments under organ culture conditions is, to date, the only method which allows essential three-dimensional cellular interactions to be maintained under conditions which permit controlled experimental manipulation in vitro. Recent modifications of explant technology, particularly within the area of fetal thymic organ culture, now allow the controlled reassociation of defined cellular subsets and manipulation of gene expression, under conditions where the functioning of both lymphoid and stromal cell types closely resembles that in vivo.

Original languageEnglish
Pages (from-to)155-163
Number of pages9
JournalJournal of Immunological Methods
Volume216
Issue number1-2
DOIs
Publication statusPublished - 1 Jul 1998

Bibliographical note

Funding Information:
This work was supported by a Wellcome Trust grant to GA and an MRC programme grant to EJJ. The authors wish to thank Katharine Partington for expert technical assistance.

Keywords

  • Organ culture
  • Stromal cells
  • Thymocyte
  • Thymus

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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