Use of 5-nitroindole-2'-deoxyribose-5'-triphosphate for labelling and detection of oligonucleotides

C L Smith, A C Simmonds, A L Hamilton, D L Martin, A G Lashford, D Loakes, F Hill, D M Brown

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The 5'-triphosphate of 5-nitroindole-2'-deoxyriboside has been shown to be a good substrate for terminal deoxynucleotidyl transferase (TdT). An antibody has been prepared for the detection of 5-nitroindole and has been used for the detection of 5-nitroindole tailed DNA both in single-stranded form and after hybridisation to a template. This is therefore a new method for the detection of nucleic acid probes.
Original languageEnglish
Pages (from-to)555-64
Number of pages10
JournalNucleosides Nucleotides & Nucleic Acids
Volume17
Issue number1-3
DOIs
Publication statusPublished - 1998

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