Ultrasound modulation of bioluminescence generated inside a turbid medium

Junaid Ahmad; Baptiste Jayet; Philip J. Hill; Melissa L. Mather; Hamid Dehghani; Stephen P. Morgan

doi:10.1117/12.2251349

Ultrasound modulation of bioluminescence generated inside a turbid medium

Junaid Ahmad, Baptiste Jayet, Philip J. Hill, Melissa L. Mather, Hamid Dehghani, Stephen P. Morgan^*

^*Corresponding author for this work

Computer Science

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

In vivo bioluminescence imaging (BLI) has poor spatial resolution owing to strong light scattering by tissue, which also affects quantitative accuracy. This paper proposes a hybrid acousto-optic imaging platform that images bioluminescence modulated at ultrasound (US) frequency inside an optically scattering medium. This produces an US modulated light within the tissue that reduces the effects of light scattering and improves the spatial resolution. The system consists of a continuously excited 3.5 MHz US transducer applied to a tissue like phantom of known optical properties embedded with bio-or chemiluminescent sources that are used to mimic in vivo experiments. Scanning US over the turbid medium modulates the luminescent sources deep inside tissue at several US scan points. These modulated signals are recorded by a photomultiplier tube and lock-in detection to generate a 1D profile. Indeed, high frequency US enables small focal volume to improve spatial resolution, but this leads to lower signal-to-noise ratio. First experimental results show that US enables localization of a small luminescent source (around 2 mm wide) deep (∼20 mm) inside a tissue phantom having a scattering coefficient of 80 cm^-1. Two sources separated by 10 mm could be resolved 20 mm inside a chicken breast.

Original language	English
Title of host publication	Photons Plus Ultrasound
Subtitle of host publication	Imaging and Sensing 2017
Editors	Alexander A. Oraevsky, Lihong V. Wang
Publisher	SPIE
ISBN (Electronic)	9781510605695
DOIs	https://doi.org/10.1117/12.2251349
Publication status	Published - 2017
Event	Photons Plus Ultrasound: Imaging and Sensing 2017 - San Francisco, United States Duration: 29 Jan 2017 → 1 Feb 2017

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	10064
ISSN (Print)	1605-7422

Conference

Conference	Photons Plus Ultrasound: Imaging and Sensing 2017
Country/Territory	United States
City	San Francisco
Period	29/01/17 → 1/02/17

Bibliographical note

Publisher Copyright:
© 2017 SPIE.

Keywords

Acousto-optic
Bioluminescence imaging (BLI)
Small animal imaging
Tissue phantom
Ultrasound (US)

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.2251349

Cite this

Ahmad, J., Jayet, B., Hill, P. J., Mather, M. L., Dehghani, H., & Morgan, S. P. (2017). Ultrasound modulation of bioluminescence generated inside a turbid medium. In A. A. Oraevsky, & L. V. Wang (Eds.), Photons Plus Ultrasound: Imaging and Sensing 2017 Article 1006454 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10064). SPIE. https://doi.org/10.1117/12.2251349

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title = "Ultrasound modulation of bioluminescence generated inside a turbid medium",

abstract = "In vivo bioluminescence imaging (BLI) has poor spatial resolution owing to strong light scattering by tissue, which also affects quantitative accuracy. This paper proposes a hybrid acousto-optic imaging platform that images bioluminescence modulated at ultrasound (US) frequency inside an optically scattering medium. This produces an US modulated light within the tissue that reduces the effects of light scattering and improves the spatial resolution. The system consists of a continuously excited 3.5 MHz US transducer applied to a tissue like phantom of known optical properties embedded with bio-or chemiluminescent sources that are used to mimic in vivo experiments. Scanning US over the turbid medium modulates the luminescent sources deep inside tissue at several US scan points. These modulated signals are recorded by a photomultiplier tube and lock-in detection to generate a 1D profile. Indeed, high frequency US enables small focal volume to improve spatial resolution, but this leads to lower signal-to-noise ratio. First experimental results show that US enables localization of a small luminescent source (around 2 mm wide) deep (∼20 mm) inside a tissue phantom having a scattering coefficient of 80 cm-1. Two sources separated by 10 mm could be resolved 20 mm inside a chicken breast.",

keywords = "Acousto-optic, Bioluminescence imaging (BLI), Small animal imaging, Tissue phantom, Ultrasound (US)",

author = "Junaid Ahmad and Baptiste Jayet and Hill, {Philip J.} and Mather, {Melissa L.} and Hamid Dehghani and Morgan, {Stephen P.}",

note = "Publisher Copyright: {\textcopyright} 2017 SPIE.; Photons Plus Ultrasound: Imaging and Sensing 2017 ; Conference date: 29-01-2017 Through 01-02-2017",

year = "2017",

doi = "10.1117/12.2251349",

language = "English",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

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Ahmad, J, Jayet, B, Hill, PJ, Mather, ML, Dehghani, H & Morgan, SP 2017, Ultrasound modulation of bioluminescence generated inside a turbid medium. in AA Oraevsky & LV Wang (eds), Photons Plus Ultrasound: Imaging and Sensing 2017., 1006454, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10064, SPIE, Photons Plus Ultrasound: Imaging and Sensing 2017, San Francisco, United States, 29/01/17. https://doi.org/10.1117/12.2251349

Ultrasound modulation of bioluminescence generated inside a turbid medium. / Ahmad, Junaid; Jayet, Baptiste; Hill, Philip J. et al.
Photons Plus Ultrasound: Imaging and Sensing 2017. ed. / Alexander A. Oraevsky; Lihong V. Wang. SPIE, 2017. 1006454 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10064).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Ultrasound modulation of bioluminescence generated inside a turbid medium

AU - Ahmad, Junaid

AU - Jayet, Baptiste

AU - Hill, Philip J.

AU - Mather, Melissa L.

AU - Dehghani, Hamid

AU - Morgan, Stephen P.

PY - 2017

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N2 - In vivo bioluminescence imaging (BLI) has poor spatial resolution owing to strong light scattering by tissue, which also affects quantitative accuracy. This paper proposes a hybrid acousto-optic imaging platform that images bioluminescence modulated at ultrasound (US) frequency inside an optically scattering medium. This produces an US modulated light within the tissue that reduces the effects of light scattering and improves the spatial resolution. The system consists of a continuously excited 3.5 MHz US transducer applied to a tissue like phantom of known optical properties embedded with bio-or chemiluminescent sources that are used to mimic in vivo experiments. Scanning US over the turbid medium modulates the luminescent sources deep inside tissue at several US scan points. These modulated signals are recorded by a photomultiplier tube and lock-in detection to generate a 1D profile. Indeed, high frequency US enables small focal volume to improve spatial resolution, but this leads to lower signal-to-noise ratio. First experimental results show that US enables localization of a small luminescent source (around 2 mm wide) deep (∼20 mm) inside a tissue phantom having a scattering coefficient of 80 cm-1. Two sources separated by 10 mm could be resolved 20 mm inside a chicken breast.

AB - In vivo bioluminescence imaging (BLI) has poor spatial resolution owing to strong light scattering by tissue, which also affects quantitative accuracy. This paper proposes a hybrid acousto-optic imaging platform that images bioluminescence modulated at ultrasound (US) frequency inside an optically scattering medium. This produces an US modulated light within the tissue that reduces the effects of light scattering and improves the spatial resolution. The system consists of a continuously excited 3.5 MHz US transducer applied to a tissue like phantom of known optical properties embedded with bio-or chemiluminescent sources that are used to mimic in vivo experiments. Scanning US over the turbid medium modulates the luminescent sources deep inside tissue at several US scan points. These modulated signals are recorded by a photomultiplier tube and lock-in detection to generate a 1D profile. Indeed, high frequency US enables small focal volume to improve spatial resolution, but this leads to lower signal-to-noise ratio. First experimental results show that US enables localization of a small luminescent source (around 2 mm wide) deep (∼20 mm) inside a tissue phantom having a scattering coefficient of 80 cm-1. Two sources separated by 10 mm could be resolved 20 mm inside a chicken breast.

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KW - Bioluminescence imaging (BLI)

KW - Small animal imaging

KW - Tissue phantom

KW - Ultrasound (US)

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DO - 10.1117/12.2251349

M3 - Conference contribution

AN - SCOPUS:85018927730

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Photons Plus Ultrasound

A2 - Oraevsky, Alexander A.

A2 - Wang, Lihong V.

PB - SPIE

T2 - Photons Plus Ultrasound: Imaging and Sensing 2017

Y2 - 29 January 2017 through 1 February 2017

ER -

Ultrasound modulation of bioluminescence generated inside a turbid medium

Abstract

Publication series

Conference

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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Cite this