tRNA 3' processing in yeast involves tRNase Z, Rex1, and Rrp6

Ewa Skowronek; Pawel Grzechnik; Bettina Späth; Anita Marchfelder; Joanna Kufel

doi:10.1261/rna.041467.113

tRNA 3' processing in yeast involves tRNase Z, Rex1, and Rrp6

Ewa Skowronek, Pawel Grzechnik, Bettina Späth, Anita Marchfelder, Joanna Kufel

Biosciences

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26 Citations (Scopus)

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Abstract

Mature tRNA 3' ends in the yeast Saccharomyces cerevisiae are generated by two pathways: endonucleolytic and exonucleolytic. Although two exonucleases, Rex1 and Rrp6, have been shown to be responsible for the exonucleolytic trimming, the identity of the endonuclease has been inferred from other systems but not confirmed in vivo. Here, we show that the yeast tRNA 3' endonuclease tRNase Z, Trz1, is catalyzing endonucleolytic tRNA 3' processing. The majority of analyzed tRNAs utilize both pathways, with a preference for the endonucleolytic one. However, 3'-end processing of precursors with long 3' trailers depends to a greater extent on Trz1. In addition to its function in the nucleus, Trz1 processes the 3' ends of mitochondrial tRNAs, contributing to the general RNA metabolism in this organelle.

Original language	English
Pages (from-to)	115-30
Number of pages	16
Journal	RNA
Volume	20
Issue number	1
Early online date	18 Nov 2013
DOIs	https://doi.org/10.1261/rna.041467.113
Publication status	Published - Jan 2014

Keywords

Cell Nucleus
Endoribonucleases
Exoribonucleases
Exosome Multienzyme Ribonuclease Complex
Gene Silencing
Metabolic Networks and Pathways
Mitochondria
Organisms, Genetically Modified
RNA
RNA 3' End Processing
RNA, Transfer
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Journal Article
tRNA
tRNase Z
3′ -end processing
exonucleases

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title = "tRNA 3' processing in yeast involves tRNase Z, Rex1, and Rrp6",

abstract = "Mature tRNA 3' ends in the yeast Saccharomyces cerevisiae are generated by two pathways: endonucleolytic and exonucleolytic. Although two exonucleases, Rex1 and Rrp6, have been shown to be responsible for the exonucleolytic trimming, the identity of the endonuclease has been inferred from other systems but not confirmed in vivo. Here, we show that the yeast tRNA 3' endonuclease tRNase Z, Trz1, is catalyzing endonucleolytic tRNA 3' processing. The majority of analyzed tRNAs utilize both pathways, with a preference for the endonucleolytic one. However, 3'-end processing of precursors with long 3' trailers depends to a greater extent on Trz1. In addition to its function in the nucleus, Trz1 processes the 3' ends of mitochondrial tRNAs, contributing to the general RNA metabolism in this organelle.",

keywords = "Cell Nucleus, Endoribonucleases, Exoribonucleases, Exosome Multienzyme Ribonuclease Complex, Gene Silencing, Metabolic Networks and Pathways, Mitochondria, Organisms, Genetically Modified, RNA, RNA 3' End Processing, RNA, Transfer, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Journal Article, tRNA, tRNase Z, 3′ -end processing, exonucleases",

author = "Ewa Skowronek and Pawel Grzechnik and Bettina Sp{\"a}th and Anita Marchfelder and Joanna Kufel",

year = "2014",

month = jan,

doi = "10.1261/rna.041467.113",

language = "English",

volume = "20",

pages = "115--30",

journal = "RNA",

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T1 - tRNA 3' processing in yeast involves tRNase Z, Rex1, and Rrp6

AU - Skowronek, Ewa

AU - Grzechnik, Pawel

AU - Späth, Bettina

AU - Marchfelder, Anita

AU - Kufel, Joanna

PY - 2014/1

Y1 - 2014/1

N2 - Mature tRNA 3' ends in the yeast Saccharomyces cerevisiae are generated by two pathways: endonucleolytic and exonucleolytic. Although two exonucleases, Rex1 and Rrp6, have been shown to be responsible for the exonucleolytic trimming, the identity of the endonuclease has been inferred from other systems but not confirmed in vivo. Here, we show that the yeast tRNA 3' endonuclease tRNase Z, Trz1, is catalyzing endonucleolytic tRNA 3' processing. The majority of analyzed tRNAs utilize both pathways, with a preference for the endonucleolytic one. However, 3'-end processing of precursors with long 3' trailers depends to a greater extent on Trz1. In addition to its function in the nucleus, Trz1 processes the 3' ends of mitochondrial tRNAs, contributing to the general RNA metabolism in this organelle.

AB - Mature tRNA 3' ends in the yeast Saccharomyces cerevisiae are generated by two pathways: endonucleolytic and exonucleolytic. Although two exonucleases, Rex1 and Rrp6, have been shown to be responsible for the exonucleolytic trimming, the identity of the endonuclease has been inferred from other systems but not confirmed in vivo. Here, we show that the yeast tRNA 3' endonuclease tRNase Z, Trz1, is catalyzing endonucleolytic tRNA 3' processing. The majority of analyzed tRNAs utilize both pathways, with a preference for the endonucleolytic one. However, 3'-end processing of precursors with long 3' trailers depends to a greater extent on Trz1. In addition to its function in the nucleus, Trz1 processes the 3' ends of mitochondrial tRNAs, contributing to the general RNA metabolism in this organelle.

KW - Cell Nucleus

KW - Endoribonucleases

KW - Exoribonucleases

KW - Exosome Multienzyme Ribonuclease Complex

KW - Gene Silencing

KW - Metabolic Networks and Pathways

KW - Mitochondria

KW - Organisms, Genetically Modified

KW - RNA

KW - RNA 3' End Processing

KW - RNA, Transfer

KW - Saccharomyces cerevisiae

KW - Saccharomyces cerevisiae Proteins

KW - Journal Article

KW - tRNA

KW - tRNase Z

KW - 3′ -end processing

KW - exonucleases

U2 - 10.1261/rna.041467.113

DO - 10.1261/rna.041467.113

M3 - Article

C2 - 24249226

SN - 1355-8382

VL - 20

SP - 115

EP - 130

JO - RNA

JF - RNA

IS - 1

ER -

tRNA 3' processing in yeast involves tRNase Z, Rex1, and Rrp6

Abstract

Keywords

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