Transcriptional responses in neonate and adult Daphnia magna in relation to relative susceptibility to genotoxicants

Little information is available on the responses of lower animals to genotoxic chemicals or on their sensitivity for detecting genotoxic chemicals, especially at different life-stages, despite the established use of the water flea Daphnia magna in ecotoxicity testing. Comet assay methodology was developed and applied to daphnid cells but only limited, non-statistically significant responses to the genotoxicants sodium dichromate (0.2-1 mu M), chrysoidine (0.1-2 mu M), and mixtures of benzo-a-pyrene (BaP) and sodium dichromate were found (from 0.01 mu M BaP & 0.1 mu M sodium dichromate to 0.25 mu M BaP & 0.75 mu M sodium dichromate). Transcriptomic analyses using Agilent D. magna oligonucleotide microarrays were undertaken to assess the effect of a mixture of sodium dichromate and BaP (designed to produce both adducted and oxidised DNA) on gene transcription. Neonates (