Tramtrack69 interacts with the dMi-2 subunit of the Drosophila NuRD chromatin remodelling complex

C M Murawsky, A Brehm, P Badenhorst, N Lowe, P B Becker, A A Travers

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53 Citations (Scopus)


dMi-2, the ATPase subunit of the Drosophila nucleosome remodelling and histone deacetylation (dNuRD) complex, was identified in a two-hybrid screen as an interacting partner of the transcriptional repressor, Tramtrack69 (Ttk69). A short region of Ttk69 is sufficient to mediate this interaction. Ttk69, but not the Ttk88 isoform, co-purifies with the dNuRD complex isolated from embryo extracts. dMi-2 and Ttk69 co-immunoprecipitate from embryonic extracts, indicating that they can associate in vivo. Both dMi-2 and Ttk69 co-localize at a number of discrete sites on polytene chromosomes, showing that they bind common target loci. We also demonstrate that dMi-2 and Ttk interact genetically, indicating a functional interaction in vivo. We propose that Ttk69 represses some target genes by remodelling chromatin structure through the recruitment of the dNuRD complex.

Original languageEnglish
Pages (from-to)1089-94
Number of pages6
JournalEMBO Reports
Issue number12
Publication statusPublished - Dec 2001


  • Adenosine Triphosphatases
  • Animals
  • Autoantigens
  • Blotting, Western
  • Carrier Proteins
  • Chromatin
  • Drosophila
  • Drosophila Proteins
  • Gene Expression Regulation
  • Histone Deacetylases
  • Mi-2 Nucleosome Remodeling and Deacetylase Complex
  • Protein Binding
  • Protein Subunits
  • Repressor Proteins
  • Two-Hybrid System Techniques
  • Yeasts


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