Abstract
Each sensory organ of the Drosophila peripheral nervous system is derived from a single sensory organ precursor cell (SOP). These originate in territories defined by expression of the proneural genes of the Achaete-Scute complex (AS-C). Formation of ectopic sensilla outside these regions is prevented by transcriptional repression of proneural genes. We demonstrate that the BTB/POZ-domain transcriptional repressor Tramtrack (Ttk) co-operates in this repression. Ttk is expressed ubiquitously, except in proneural clusters and SOPs. Ttk over-expression represses proneural genes and sensilla formation. Loss of Ttk enhances bristle-promoting mutants. Using neural repression as an assay, we dissected functional domains of Ttk, confirming the importance of the bric-à-brac-tramtrack-broad complex (BTB) motif. We show that the Ttk BTB domain is a protein-protein interaction motif mediating tetramer formation.
Original language | English |
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Pages (from-to) | 87-101 |
Number of pages | 15 |
Journal | Mechanisms of Development |
Volume | 117 |
Issue number | 1-2 |
Publication status | Published - Sept 2002 |
Bibliographical note
Copyright 2002 Elsevier Science Ireland Ltd.Keywords
- Animals
- Binding Sites
- Drosophila
- Drosophila Proteins
- Female
- Gene Expression Regulation, Developmental
- Genes, Insect
- Male
- Mutagenesis
- Peripheral Nerves
- Protein Structure, Tertiary
- Recombinant Fusion Proteins
- Repressor Proteins
- Sense Organs