Each sensory organ of the Drosophila peripheral nervous system is derived from a single sensory organ precursor cell (SOP). These originate in territories defined by expression of the proneural genes of the Achaete-Scute complex (AS-C). Formation of ectopic sensilla outside these regions is prevented by transcriptional repression of proneural genes. We demonstrate that the BTB/POZ-domain transcriptional repressor Tramtrack (Ttk) co-operates in this repression. Ttk is expressed ubiquitously, except in proneural clusters and SOPs. Ttk over-expression represses proneural genes and sensilla formation. Loss of Ttk enhances bristle-promoting mutants. Using neural repression as an assay, we dissected functional domains of Ttk, confirming the importance of the bric-à-brac-tramtrack-broad complex (BTB) motif. We show that the Ttk BTB domain is a protein-protein interaction motif mediating tetramer formation.
|Number of pages||15|
|Journal||Mechanisms of Development|
|Publication status||Published - Sept 2002|
Bibliographical noteCopyright 2002 Elsevier Science Ireland Ltd.
- Binding Sites
- Drosophila Proteins
- Gene Expression Regulation, Developmental
- Genes, Insect
- Peripheral Nerves
- Protein Structure, Tertiary
- Recombinant Fusion Proteins
- Repressor Proteins
- Sense Organs