TY - JOUR
T1 - The upper respiratory tract microbiome of indigenous Orang Asli in north-eastern Peninsular Malaysia
AU - Cleary, David
AU - Morris, Denise
AU - Anderson, Rebecca
AU - Jones, Jessica
AU - Alattraqchi, Ahmed Ghazi
AU - Rahman, Nor Iza
AU - Ismail, Salwani
AU - Razali, Mohamad
AU - Amin, Rahmah Mohd
AU - Aziz, Aniza Abd
AU - Esa, Nor Kamarruzaman
AU - Amiruddin, Salman
AU - Chew, Ching Hoong
AU - Amat Simin, Mohamad Hafis
AU - Abdullah, Ramle
AU - Yeo, Chew Chieng
AU - Clarke, Stuart
PY - 2021/12
Y1 - 2021/12
N2 - Much microbiome research has focused on populations that are predominantly of European descent, and from narrow demographics that do not capture the socio-economic and lifestyle differences which impact human health. Here we examined the airway microbiomes of the Orang Asli, the indigenous peoples of Malaysia. A total of 130 participants were recruited from two sites in the north-eastern state of Terengganu in Peninsular Malaysia. Using 16S rRNA sequencing, the nasal microbiome was significantly more diverse in those aged 5–17 years compared to 50+ years (p = 0.023) and clustered by age (PERMANOVA analysis of the Bray–Curtis distance, p = 0.001). Hierarchical clustering of Bray–Curtis dissimilarity scores revealed six microbiome clusters. The largest cluster (n = 28; 35.4%) had a marked abundance of Corynebacterium. In the oral microbiomes Streptococcus, Neisseria and Haemophilus were dominant. Using conventional microbiology, high levels of Staphylococcus aureus carriage were observed, particularly in the 18–65 age group (n = 17/36; 47.2% 95% CI: 30.9–63.5). The highest carriage of pneumococci was in the
AB - Much microbiome research has focused on populations that are predominantly of European descent, and from narrow demographics that do not capture the socio-economic and lifestyle differences which impact human health. Here we examined the airway microbiomes of the Orang Asli, the indigenous peoples of Malaysia. A total of 130 participants were recruited from two sites in the north-eastern state of Terengganu in Peninsular Malaysia. Using 16S rRNA sequencing, the nasal microbiome was significantly more diverse in those aged 5–17 years compared to 50+ years (p = 0.023) and clustered by age (PERMANOVA analysis of the Bray–Curtis distance, p = 0.001). Hierarchical clustering of Bray–Curtis dissimilarity scores revealed six microbiome clusters. The largest cluster (n = 28; 35.4%) had a marked abundance of Corynebacterium. In the oral microbiomes Streptococcus, Neisseria and Haemophilus were dominant. Using conventional microbiology, high levels of Staphylococcus aureus carriage were observed, particularly in the 18–65 age group (n = 17/36; 47.2% 95% CI: 30.9–63.5). The highest carriage of pneumococci was in the
U2 - 10.1038/s41522-020-00173-5
DO - 10.1038/s41522-020-00173-5
M3 - Article
SN - 2055-5008
VL - 7
JO - npj Biofilms and Microbiomes
JF - npj Biofilms and Microbiomes
IS - 1
M1 - 1
ER -
