The structurally effect of surface coated rhamnogalacturonan I on response of the osteoblast-like cell line SaOS-2

Rikke Svava, Katarzyna Gurzawska, Yu Yihau, Kenneth Brian Haugshøj, Kai Dirscherl, Steven B Levery, Niklas Rye Jørgensen, Klaus Gotfredsen, Iben Damager, Peter Ulvskov, Bodil Jørgensen

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5 Citations (Scopus)


Osseointegration is important when implants are inserted into the bone and can be improved by biochemical surface coating of the implant. In this paper enzymatically modified rhamnogalacturonan I (RG-I) from apple and lupin was used for biochemical coating of aminated surfaces and the importance of the quality of RG-I, the nature of the binding, the fine structure of RG-I, and its effect on SaOS-2 cell line cultured on coated surfaces was investigated. SaOS-2 cells are osteoblast-like cells and a well-established in vitro model of bone-matrix forming osteoblasts. Purification by gel filtration could remove small fragments of galacturonic acid (GalA) and binding studies showed that the purity of the RG-I molecules was important for the quality of the coating. The structure of RG-I and osteoblast-like cells' viability were positively correlated so that high content of 1,4-linked galactose (Gal) and a low content of arabinose in the RG-I molecules favored cell viability. These results indicate that coating of implants with RG-I affect osseointegration positively.

Original languageEnglish
Pages (from-to)1961-71
Number of pages11
JournalJournal of Biomedical Materials Research Part A
Issue number6
Early online date12 Jul 2013
Publication statusPublished - 1 Jun 2014


  • Cell Line
  • Cell Survival
  • Coated Materials, Biocompatible
  • Dental Implants
  • Humans
  • Lupinus
  • Malus
  • Osteoblasts
  • Pectins
  • Journal Article
  • Research Support, Non-U.S. Gov't
  • dental implant
  • polysaccharide
  • surface modification
  • SaOS‐2 cell line
  • Osseointegration


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