The stimulation of IL-2 production by anti-rheumatic drugs

J A Oben, G R Wallace, B M Chain, J C Foreman

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12 Citations (Scopus)

Abstract

IL-2 release from mouse splenocytes was measured by assaying the IL-2 on an IL-2-dependent cytotoxic T-lymphocyte line in culture (CTLL). Proliferation of the CTLL cells was monitored indirectly with the dye thiazolyl blue. The slow-acting anti-rheumatic drug auranofin at concentrations below 0.1 microM potentiated concanavalin A (Con A)-induced IL-2 release. Similar potentiation of Con A-induced IL-2 release was obtained with D-penicillamine, 1 microM-1 mM, and with the angiotensin-converting enzyme-inhibitor captopril, 10 nM-1 microM. Potentiation of Con A-induced IL-2 release was obtained with concentrations of the drugs likely to be achieved in vivo during therapy. Auranofin but not D-penicillamine and captopril inhibited Con A-induced IL-2 release at high concentrations (greater than 0.3 microM).

Original languageEnglish
Pages (from-to)328-32
Number of pages5
JournalImmunology
Volume67
Issue number3
Publication statusPublished - Jul 1989

Keywords

  • Animals
  • Auranofin
  • Captopril
  • Cells, Cultured
  • Interleukin-2
  • Mice
  • Penicillamine
  • Spleen

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