The selection of high-producing cell lines using flow sytometry and cell sorting

S Carroll, Mohamed Al-Rubeai

Research output: Contribution to journalArticle

59 Citations (Scopus)


The selection of high-producing cell lines is usually time-consuming and labour-intensive. Following transfection, high-producing cells are selected using limiting dilution cloning to prevent non- and low-producing cells from outgrowing high-producing cells, a process that normally takes > 3 months. During this time, the cells have to be screened occasionally to ensure stability of the selected clone. Several new methods for selecting and screening cells using flow cytometry and cell sorting have recently been developed; these include gel microdrop technology, which encapsulates the cells in gelatine beads, and matrix-based secretion assays. This paper reviews these techniques for selecting high-producing cell lines and isolating rare cells.
Original languageEnglish
Pages (from-to)1821-1829
Number of pages9
JournalExpert Opinion on Biological Therapy
Issue number11
Publication statusPublished - 1 Jan 2004


  • matrix-based secretion assays
  • cell culture
  • high producers
  • flow cytometry
  • gel microdrop
  • biopharmaceuticals


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