The Role of Topoisomerase II in DNA Repair and Recombination in Arabidopsis thaliana

Marina Martinez-Garcia, Charles I White, F Chris H Franklin, Eugenio Sanchez-Moran

Research output: Contribution to journalArticlepeer-review

Abstract

DNA entanglements and supercoiling arise frequently during normal DNA metabolism. DNA topoisomerases are highly conserved enzymes that resolve the topological problems that these structures create. Topoisomerase II (TOPII) releases topological stress in DNA by removing DNA supercoils through breaking the two DNA strands, passing a DNA duplex through the break and religating the broken strands. TOPII performs key DNA metabolic roles essential for DNA replication, chromosome condensation, heterochromatin metabolism, telomere disentanglement, centromere decatenation, transmission of crossover (CO) interference, interlock resolution and chromosome segregation in several model organisms. In this study, we reveal the endogenous role of Arabidopsis thaliana TOPII in normal root growth and cell cycle, and mitotic DNA repair via homologous recombination. Additionally, we show that the protein is required for meiotic DSB repair progression, but not for CO formation. We propose that TOPII might promote mitotic HR DNA repair by relieving stress needed for HR strand invasion and D-loop formation.

Original languageEnglish
Article number13115
JournalInternational Journal of Molecular Sciences
Volume22
Issue number23
DOIs
Publication statusPublished - 4 Dec 2021

Keywords

  • Arabidopsis/cytology
  • Arabidopsis Proteins/genetics
  • Chromosome Segregation
  • Chromosomes, Plant
  • DNA Breaks, Double-Stranded
  • DNA Repair/physiology
  • DNA Replication
  • DNA Topoisomerases, Type II/genetics
  • Gamma Rays
  • Homologous Recombination
  • Meiosis
  • Mitomycin/pharmacology
  • Mutation

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