The P5-type ATPase ATP13A1 modulates major histocompatibility complex I-related protein 1 (MR1)-mediated antigen presentation

Corinna A Kulicke, Erica De Zan, Zeynep Hein, Claudia Gonzalez-Lopez, Swapnil Ghanwat, Natacha Veerapen, Gurdyal S Besra, Paul Klenerman, John C Christianson, Sebastian Springer, Sebastian Nijman, Vincenzo Cerundolo, Mariolina Salio

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The monomorphic antigen-presenting molecule major histocompatibility complex-I-related protein 1 (MR1) presents small-molecule metabolites to mucosal-associated invariant T (MAIT) cells. The MR1-MAIT cell axis has been implicated in a variety of infectious and noncommunicable diseases, and recent studies have begun to develop an understanding of the molecular mechanisms underlying this specialized antigen presentation pathway. However, proteins regulating MR1 folding, loading, stability, and surface expression remain to be identified. Here, we performed a gene trap screen to discover novel modulators of MR1 surface expression through insertional mutagenesis of an MR1-overexpressing clone derived from the near-haploid human cell line HAP1 (HAP1.MR1). The most significant positive regulators identified included β2-microglobulin, a known regulator of MR1 surface expression, and ATP13A1, a P5-type ATPase in the endoplasmic reticulum (ER) not previously known to be associated with MR1-mediated antigen presentation. CRISPR/Cas9-mediated knockout of ATP13A1 in both HAP1.MR1 and THP-1 cell lines revealed a profound reduction in MR1 protein levels and a concomitant functional defect specific to MR1-mediated antigen presentation. Collectively, these data are consistent with the ER-resident ATP13A1 being a key posttranscriptional determinant of MR1 surface expression.

Original languageEnglish
Article number101542
JournalThe Journal of biological chemistry
Issue number2
Early online date27 Dec 2021
Publication statusPublished - Feb 2022


  • ATP13A1
  • HAP1
  • MHC I-related protein 1 (MR1)
  • MR1-restricted T cell (MR1T)
  • P5-type ATPase
  • antigen presentation
  • gene trap
  • mucosal-associated invariant T cell (MAIT)
  • protein trafficking


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